Promoter-upstream activator sequences are required for expression of the xylS gene and upper-pathway operon on the Pseudomonas TOL plasmid

Stimulation of transcription from the Pseudomonas TOL plasmid xylS gene promoter (Ps) and the upper-pathway operon promoter (Pu) is dependent on the positive regulator protein XylR activated by an effector molecule such as 3-cholorotoluene, and on RpoN, an RNA polymerase sigma factor. Mutational ana...

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Bibliographic Details
Published inMolecular microbiology Vol. 4; no. 9; p. 1551
Main Authors Holtel, A, Abril, M A, Marques, S, Timmis, K N, Ramos, J L
Format Journal Article
LanguageEnglish
Published England 01.09.1990
Subjects
Base Sequence
DNA Mutational Analysis
Gene Expression Regulation, Bacterial
Genes, Bacterial
Genes, Regulator
Molecular Sequence Data
Operon
Plasmids
Promoter Regions, Genetic
Pseudomonas - genetics
Pseudomonas - metabolism
Sequence Homology, Nucleic Acid
Toluene - metabolism
Transcription, Genetic
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Summary:Stimulation of transcription from the Pseudomonas TOL plasmid xylS gene promoter (Ps) and the upper-pathway operon promoter (Pu) is dependent on the positive regulator protein XylR activated by an effector molecule such as 3-cholorotoluene, and on RpoN, an RNA polymerase sigma factor. Mutational analysis of the Ps and Pu promoters showed that upstream activator sequences located between -110 and -218bp upstream of the main transcription initiation point are required for regulated expression from these promoters. A search for homologous nucleotide sequences in the -110 to -218bp region in Pu and Ps revealed conserved sequences that may act as putative recognition sequences for the XylR protein. Ps and Pu exhibit another well-conserved region at around -50bp, which is homologous to corresponding sites in other RpoN-dependent promoters and may constitute a binding site for integration host factor (IHF).
ISSN:0950-382X
DOI:10.1111/j.1365-2958.1990.tb02066.x