Effect of metastasis suppressor 1 on H1299 cells and its clinical significance in non-small cell lung cancer

• Published in: Oncology reports Vol. 36; no. 5; pp. 2814 - 2822
• Main Authors: Zhao, Heyan, Xue, Jianhua, Liu, Junhua, Liu, Yifei, Cheng, Yinan
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.11.2016; Spandidos Publications; Spandidos Publications UK Ltd
• Subjects: adenocarcinoma; Adult; Aged; Breast cancer; Carcinoma, Non-Small-Cell Lung - genetics; Carcinoma, Non-Small-Cell Lung - pathology; Care and treatment; Cell Line, Tumor; Cell Movement - genetics; Cell Proliferation - genetics; Clinical significance; Development and progression; Female; Gastric cancer; Gender; Gene expression; Gene Expression Regulation, Neoplastic; Genetic aspects; H1299; Health aspects; Humans; Lung cancer; Lung cancer, Non-small cell; Male; Medical prognosis; Medical research; Metastasis; Microfilament Proteins - biosynthesis; Microfilament Proteins - genetics; Middle Aged; MTSS1; Neoplasm Invasiveness - genetics; Neoplasm Proteins - biosynthesis; Neoplasm Proteins - genetics; non-small cell lung cancer; Patients; Polymerase chain reaction; Prognosis; Prostate; Proteins; RNA, Messenger - biosynthesis; Signal transduction; Squamous cell carcinoma; Studies; Tumor suppressor genes; Tumors; United States > US
• ISSN: 1021-335X; 1791-2431
• DOI: 10.3892/or.2016.5081

The present study aimed to investigate the effect of metastasis suppressor 1 (MTSS1) on the proliferation, migration and invasion of human H1299 non-small cell lung cancer cells and its clinical significance in non-small cell lung cancer. The target gene MTSS1-overexpressing lentivirus (LV-MTSS1) was transfected into H1299 cells and expression of MTSS1 was detected at the mRNA and protein levels. Cell Counting Kit-8, wound healing and Transwell assays revealed that the migration and invasion activities were significantly suppressed by MTSS1, but that it had no effect on cell proliferation. In addition, MTSS1 expression in tissue microarrays including samples from 223 cases of non-small cell lung cancer was tested by immunohistochemistry to explore the correlation between MTSS1 and clinicopathological characteristics and prognosis. MTSS1 suppressed H1299 cell migration and invasion, and its expression level can be used as a new independent factor for determining the prognosis of non-small cell lung cancer.