Biologic activity of an idiotype-bearing suppressor T cell factor produced by a long-term T cell hybridoma

• Published in: The Journal of immunology (1950) Vol. 128; no. 1; pp. 49 - 53
• Main Authors: Takaoki, M, Sy, MS, Whitaker, B, Nepom, J, Finberg, R, Germain, RN, Nisonoff, A, Benacerraf, B, Greene, MI
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 01.01.1982
• Subjects: Animals; Cells, Cultured; Cross Reactions; Cytotoxicity, Immunologic; Female; Hybridomas - immunology; Hybridomas - secretion; Immunoglobulin Heavy Chains - genetics; Immunoglobulin Heavy Chains - immunology; Immunoglobulin Idiotypes - immunology; Lymphokines - biosynthesis; Male; Mice; Mice, Inbred A; Mice, Inbred AKR; Mice, Inbred BALB C; p-Azobenzenearsonate - immunology; Suppressor Factors, Immunologic; T-Lymphocytes - immunology; T-Lymphocytes, Regulatory - immunology; Time Factors
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.128.1.49

Biologic activities and immunochemical characteristics of an azobenzenearsonate- (ABA)specific suppressor T cell factor produced by a longterm T cell hybridoma, F12, were studied. In vivo administration of F12 culture supernatant resulted in the suppression of ABA-specific delayed-type hypersensitivity (DTH) responses and the inhibition of priming for ABA-specific cytotoxic T lymphocyte responses. Moreover, F12 induced a second set of suppressor cells that act in the efferent phase of DTH. The active material in the F12 culture supernatant expressed major cross-reactive idiotypic (CRI) determinants of anti-ABA antibodies of A/J mice and I-J subregion-coded specificities but not express determinants of immunoglobulin constant regions. These results demonstrated that F12 is a functioning hybrid cell line of the first-order suppressor T cell subset.