Low dose radiation overcomes diabetes-induced suppression of hippocampal neuronal cell proliferation in rats

We investigated the effect of low dose radiation on diabetes induced suppression of neurogenesis in the hippocampal dentate gyrus of rat. After 0.01 Gy, 0.1 Gy, 1 Gy and 10 Gy radiation was delivered, the dentate gyrus of hippocampus of streptozotocin (STZ)-induced diabetic rats were evaluated using...

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Published inJournal of Korean medical science Vol. 21; no. 3; pp. 500 - 505
Main Authors Kang, Jin Oh, Kim, Sang Ki, Hong, Seong Eon, Lee, Taeck Hyun, Kim, Chang Ju
Format Journal Article
LanguageEnglish
Published Korea (South) The Korean Academy of Medical Sciences 01.06.2006
Subjects
Animals
Apoptosis
Bromodeoxyuridine - pharmacology
Caspase 3 - metabolism
Cell Proliferation
Dentate Gyrus - drug effects
Dentate Gyrus - radiation effects
Diabetes Mellitus, Experimental - radiotherapy
Hippocampus - cytology
Hippocampus - metabolism
Hippocampus - radiation effects
In Situ Nick-End Labeling
Male
Neurons - metabolism
Original
Radiotherapy - methods
Rats
Rats, Sprague-Dawley
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Summary:We investigated the effect of low dose radiation on diabetes induced suppression of neurogenesis in the hippocampal dentate gyrus of rat. After 0.01 Gy, 0.1 Gy, 1 Gy and 10 Gy radiation was delivered, the dentate gyrus of hippocampus of streptozotocin (STZ)-induced diabetic rats were evaluated using immunohistochemistry for 5-bromo-2-deoxyuridine (BrdU), caspase-3, and terminal deoxynucleotidyl transferase-mediated nick end-labeling (TUNEL) staining. The number of BrdU positive cells in the non-diabetic rats, diabetic rats without radiation, diabetic rats with 0.01 Gy radiation, diabetic rats with 0.1 Gy radiation, diabetic rats with 1 Gy radiation and diabetic rats with 10 Gy radiation were 55.4+/-8.5/mm2, 33.3+/-6.4/mm2, 67.7+/-10.5/mm2, 66.6+/-10.0/mm2, 23.5+/-6.3/mm2 and 14.3+/-7.2/mm2, respectively. The number of caspase-3 positive cells was 132.6+/-37.4/mm2, 378.6+/-99.1/mm2, 15.0+/-2.8/mm2, 57.1+/-16.9/mm2, 191.8+/-44.8/mm2 and 450.4+/-58.3/mm2, respectively. The number of TUNEL-positive cells was 24.5+/-2.0/mm2, 21.7+/-4.0/mm2, 20.4+/-2.0/mm2, 18.96+/-2.1/mm2, 58.3+/-7.9/mm2, and 106.0+/-9.8/mm2, respectively. These results suggest low doses of radiation paradoxically improved diabetes induced neuronal cell suppression in the hippocampal dentate gyrus of rat.
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ISSN:1011-8934
1598-6357
DOI:10.3346/jkms.2006.21.3.500