The complete nucleotide sequence and its organization of the genome of Barley yellow dwarf virus-GAV

• Published in: Science China. Life sciences Vol. 47; no. 2; pp. 175 - 182
• Main Authors: Jin, Zhibo, Wang, Xifeng, Chang, Shengjun, Zhou, Guanghe
• Format: Journal Article
• Language: English
• Published: China Springer Nature B.V 01.04.2004; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China
• Subjects: Barley yellow dwarf virus; Base Sequence; Cloning, Molecular; DNA, Complementary - genetics; DNA, Viral - chemistry; DNA, Viral - genetics; Dwarf disease; Genomics; Hordeum - virology; Luteovirus - classification; Luteovirus - genetics; Molecular Sequence Data; Nucleotide sequence; Open reading frames; Phylogeny; Presenilin 1; Sequence Alignment; Barley yellow dwarf virus-GAV (BYDV-GAV); genomic organization; taxonomy
• ISSN: 1006-9305; 1674-7305; 1862-2798; 1869-1889
• DOI: 10.1360/03yc0076

The complete nucleotide sequence of genomic RNA of BYDV-GAV was determined. It comprised 5685 nucleotides and contained six open reading frames and four un-translated regions. The size and organization of BYDV-GAV genome were similar to those of BYDV PAV-aus. The nucleotide and deduced amino acid sequences of the six ORFs were aligned and compared with those of other luteoviruses. The results showed that there was a high degree of identity between BYDV-GAV and MAV-PS1 in all ORFs except ORF5 and ORF6, which had only 87.4% and 70.2% identities respectively. The reported genomic nucleotide sequence of MAV was shorter than that of BYDV-GAV, but the comparison of the genomic nucleotide sequences for MAV-PS1 and GAV showed 90.4% sequence identity for the same region of the genome. According to the level of sequence similarities, BYDV-GAV should be closely related to BYDV-MAV.