Proteome Analysis of Cerebrospinal Fluid in Amyotrophic Lateral Sclerosis (ALS)

• Published in: Neurochemical research Vol. 33; no. 11; pp. 2358 - 2363
• Main Authors: Brettschneider, Johannes, Mogel, Helga, Lehmensiek, Vera, Ahlert, Tino, Süssmuth, Sigurd, Ludolph, Albert C., Tumani, Hayrettin
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.11.2008; Springer Nature B.V
• Subjects: Aged; Aged, 80 and over; Amyotrophic Lateral Sclerosis - cerebrospinal fluid; Biochemistry; Biomedical and Life Sciences; Biomedicine; Cell Biology; Electrophoresis, Gel, Two-Dimensional; Enzyme-Linked Immunosorbent Assay; Female; Humans; Male; Mass Spectrometry; Middle Aged; Neurochemistry; Neurology; Neurosciences; Original Paper; Proteome; MND; Motor neuron disease; ALS; Amyotrophic lateral sclerosis; Proteome; Difference in gel electrophoresis; Cerebrospinal fluid
• DOI: 10.1007/s11064-008-9742-5
• ISSN: 0364-3190

Cerebrospinal fluid (CSF) is a promising source of biomarkers in amyotrophic lateral sclerosis (ALS). Using the two-dimensional difference in gel electrophoresis (2-D-DIGE), we compared CSF samples from patients with ALS ( n = 14) with those from normal controls ( n = 14). Protein spots that showed significant differences between patients and controls were selected for further analysis by MALDI-TOF mass spectrometry. For validation of identified spots western blot analysis and ELISA was performed. We identified 2 proteins that were upregulated and 3 proteins that were down-regulated in CSF in ALS. Of these, two proteins (Zn-alpha-2-glycoprotein and ceruloplasmin precursor protein) have not been reported in CSF of patients with ALS so far. In contrast, several other proteins (transferrin, alpha-1-antitrypsin precursor and beta-2-microglobulin) seem to be unspecifically affected in different neurological diseases and may therefore be of limited value as disease-related biochemical markers in ALS. Further evaluation of the candidate proteins identified here is necessary.