Proteome Analysis of Cerebrospinal Fluid in Amyotrophic Lateral Sclerosis (ALS)
Cerebrospinal fluid (CSF) is a promising source of biomarkers in amyotrophic lateral sclerosis (ALS). Using the two-dimensional difference in gel electrophoresis (2-D-DIGE), we compared CSF samples from patients with ALS ( n = 14) with those from normal controls ( n = 14). Protein spots that showed...
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Published in | Neurochemical research Vol. 33; no. 11; pp. 2358 - 2363 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Boston
Springer US
01.11.2008
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Cerebrospinal fluid (CSF) is a promising source of biomarkers in amyotrophic lateral sclerosis (ALS). Using the two-dimensional difference in gel electrophoresis (2-D-DIGE), we compared CSF samples from patients with ALS (
n
= 14) with those from normal controls (
n
= 14). Protein spots that showed significant differences between patients and controls were selected for further analysis by MALDI-TOF mass spectrometry. For validation of identified spots western blot analysis and ELISA was performed. We identified 2 proteins that were upregulated and 3 proteins that were down-regulated in CSF in ALS. Of these, two proteins (Zn-alpha-2-glycoprotein and ceruloplasmin precursor protein) have not been reported in CSF of patients with ALS so far. In contrast, several other proteins (transferrin, alpha-1-antitrypsin precursor and beta-2-microglobulin) seem to be unspecifically affected in different neurological diseases and may therefore be of limited value as disease-related biochemical markers in ALS. Further evaluation of the candidate proteins identified here is necessary. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0364-3190 1573-6903 |
DOI: | 10.1007/s11064-008-9742-5 |