Electron spin resonance studies of free radicals in gamma-irradiated golden hamster embryo cells: radical formation at 77 and 295 K, and radioprotective effects of vitamin C at 295 K

Formation of free radicals in golden hamster embryo (GHE) cells produced by gamma irradiation at 77 and 295 K has been studied by electron spin resonance (ESR) spectroscopy. The yields of free radicals in the gamma-irradiated frozen cells at 77 K increase linearly with increasing dose in the range f...

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Published inRadiation research Vol. 136; no. 3; p. 361
Main Authors Yoshimura, T, Matsuno, K, Miyazaki, T, Suzuki, K, Watanabe, M
Format Journal Article
LanguageEnglish
Published United States 01.12.1993
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Abstract Formation of free radicals in golden hamster embryo (GHE) cells produced by gamma irradiation at 77 and 295 K has been studied by electron spin resonance (ESR) spectroscopy. The yields of free radicals in the gamma-irradiated frozen cells at 77 K increase linearly with increasing dose in the range from 8 Gy to 1.3 kGy, suggesting that the mechanism of radical formation at a dose of 8 Gy is the same as that at 1.3 kGy. When GHE cells are irradiated with 5 kGy gamma rays at 295 K and then the ESR spectrum is measured at 77 K, a part of the organic radicals produced can be observed clearly. The organic radicals survive in GHE cells for more than 24 h at room temperature. The formation of the organic radicals by gamma irradiation is suppressed upon the addition of vitamin C to the GHE cells. When an aqueous solution of albumin (0.1 kg dm-3) is gamma-irradiated with 5 kGy at 295 K, albumin radicals are observed by ESR. The formation of the albumin radicals is suppressed drastically upon the addition of vitamin C. The efficient radioprotection of vitamin C against radical formation has been interpreted in terms of the scavenging of albumin radicals by vitamin C.
AbstractList Formation of free radicals in golden hamster embryo (GHE) cells produced by gamma irradiation at 77 and 295 K has been studied by electron spin resonance (ESR) spectroscopy. The yields of free radicals in the gamma-irradiated frozen cells at 77 K increase linearly with increasing dose in the range from 8 Gy to 1.3 kGy, suggesting that the mechanism of radical formation at a dose of 8 Gy is the same as that at 1.3 kGy. When GHE cells are irradiated with 5 kGy gamma rays at 295 K and then the ESR spectrum is measured at 77 K, a part of the organic radicals produced can be observed clearly. The organic radicals survive in GHE cells for more than 24 h at room temperature. The formation of the organic radicals by gamma irradiation is suppressed upon the addition of vitamin C to the GHE cells. When an aqueous solution of albumin (0.1 kg dm-3) is gamma-irradiated with 5 kGy at 295 K, albumin radicals are observed by ESR. The formation of the albumin radicals is suppressed drastically upon the addition of vitamin C. The efficient radioprotection of vitamin C against radical formation has been interpreted in terms of the scavenging of albumin radicals by vitamin C.
Author Suzuki, K
Yoshimura, T
Miyazaki, T
Watanabe, M
Matsuno, K
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Snippet Formation of free radicals in golden hamster embryo (GHE) cells produced by gamma irradiation at 77 and 295 K has been studied by electron spin resonance (ESR)...
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StartPage 361
SubjectTerms Animals
Ascorbic Acid - pharmacology
Cell Line
Cricetinae
Dimethyl Sulfoxide - pharmacology
Dose-Response Relationship, Radiation
Electron Spin Resonance Spectroscopy
Embryo, Mammalian - chemistry
Embryo, Mammalian - drug effects
Embryo, Mammalian - radiation effects
Free Radicals
Gamma Rays
Mesocricetus
Radiation-Protective Agents - pharmacology
Temperature
Title Electron spin resonance studies of free radicals in gamma-irradiated golden hamster embryo cells: radical formation at 77 and 295 K, and radioprotective effects of vitamin C at 295 K
URI https://www.ncbi.nlm.nih.gov/pubmed/8278577
Volume 136
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