Electron spin resonance studies of free radicals in gamma-irradiated golden hamster embryo cells: radical formation at 77 and 295 K, and radioprotective effects of vitamin C at 295 K
Formation of free radicals in golden hamster embryo (GHE) cells produced by gamma irradiation at 77 and 295 K has been studied by electron spin resonance (ESR) spectroscopy. The yields of free radicals in the gamma-irradiated frozen cells at 77 K increase linearly with increasing dose in the range f...
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Published in | Radiation research Vol. 136; no. 3; p. 361 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.12.1993
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Subjects | |
Online Access | Get more information |
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Summary: | Formation of free radicals in golden hamster embryo (GHE) cells produced by gamma irradiation at 77 and 295 K has been studied by electron spin resonance (ESR) spectroscopy. The yields of free radicals in the gamma-irradiated frozen cells at 77 K increase linearly with increasing dose in the range from 8 Gy to 1.3 kGy, suggesting that the mechanism of radical formation at a dose of 8 Gy is the same as that at 1.3 kGy. When GHE cells are irradiated with 5 kGy gamma rays at 295 K and then the ESR spectrum is measured at 77 K, a part of the organic radicals produced can be observed clearly. The organic radicals survive in GHE cells for more than 24 h at room temperature. The formation of the organic radicals by gamma irradiation is suppressed upon the addition of vitamin C to the GHE cells. When an aqueous solution of albumin (0.1 kg dm-3) is gamma-irradiated with 5 kGy at 295 K, albumin radicals are observed by ESR. The formation of the albumin radicals is suppressed drastically upon the addition of vitamin C. The efficient radioprotection of vitamin C against radical formation has been interpreted in terms of the scavenging of albumin radicals by vitamin C. |
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ISSN: | 0033-7587 |
DOI: | 10.2307/3578548 |