Photo-Fenton and TiO2 Photocatalytic Inactivation of Model Microorganisms under UV-A; Comparative Efficacy and Optimization

• Published in: Molecules (Basel, Switzerland) Vol. 28; no. 3; p. 1199
• Main Authors: Kanata, Eirini, Paspaltsis, Ioannis, Sotiriadis, Sotiris, Berberidou, Chrysanthi, Tsoumachidou, Sophia, Dafou, Dimitra, Xanthopoulos, Konstantinos, Arsenakis, Minas, Arsenakis, Athanasios, Poulios, Ioannis, Sklaviadis, Theodoros
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 26.01.2023; MDPI
• Subjects: Aqueous solutions; Bacillus stearothermophilus spores; Catalysts; Coliforms; Comparative studies; E coli; Enzymes; Free radicals; Gram-negative bacteria; Hydrogen peroxide; Inactivation; Medical wastes; microorganism inactivation; Microorganisms; MS2; Optimization; Oxidation; Oxidative stress; Pathogens; photo-Fenton; Photocatalysis; photocatalytic oxidation; Proteins; Reagents; Spores; TiO2; Titanium dioxide; Viruses
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28031199

Photocatalytic inactivation of pathogens in aqueous waste is gaining increasing attention. Several homogeneous and heterogeneous photocatalytic protocols exist using the Fenton’s reagent and TiO2, respectively. A comprehensive study of homogeneous and heterogeneous photocatalysis on a range of microorganisms will significantly establish the most efficient method. Here, we report a comparative study of TiO2- and Fe+3-based photocatalytic inactivation under UV-A of diverse microorganisms, including Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria, bacterial spores (Bacillus stearothermophilus spores) and viruses (MS2). We also present data on the optimization of TiO2 photocatalysis, including optimal catalyst concentration and H2O2 supplementation. Our results indicate that both photo-Fenton and TiO2 could be successfully applied for the management of microbial loads in liquids. Efficient microorganism inactivation is achieved with homogeneous photocatalysis (7 mg/L Fe+3, 100 mg/L H2O2, UV-A) in a shorter processing time compared to heterogeneous photocatalysis (0.5 g/L TiO2, UV-A), whereas similar or shorter processing is required when heterogenous photocatalysis is performed using microorganism-specific optimized TiO2 concentrations and H2O2 supplementation (100 mg/L); higher H2O2 concentrations further enhance the heterogenous photocatalytic inactivation efficiency. Our study provides a template protocol for the design and further application for large-scale photocatalytic approaches to inactivate pathogens in liquid biomedical waste.