Volatile Organic Compounds and Physiological Parameters as Markers of Potato (Solanum tuberosum L.) Infection with Phytopathogens

• Published in: Molecules (Basel, Switzerland) Vol. 27; no. 12; p. 3708
• Main Authors: Steglińska, Aleksandra, Pielech-Przybylska, Katarzyna, Janas, Regina, Grzesik, Mieczysław, Borowski, Sebastian, Kręgiel, Dorota, Gutarowska, Beata
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 09.06.2022; MDPI
• Subjects: Acetic acid; Alternaria tenuissima; Benzothiazole; Butanol; Chlorophyll; Disease; Disease detection; Field study; Fusarium sambucinum; Gangrene; Gas exchange; Indicators; Infections; Markers; Metabolism; Metabolites; Microorganisms; Octanol; Organic compounds; Organs; Pathogens; Pectobacterium carotovorum; Physiological effects; Physiology; phytopathogen volatiles; phytopathogens; Plant growth; potato physiology; Potatoes; seed potatoes; Seeds; soft rot; Solanum tuberosum; Solid phase methods; Trimethylbenzene; Vegetables; VOCs; Volatile compounds; Volatile organic compounds; Volatiles; Volatility
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27123708

The feasibility of early disease detection in potato seeds storage monitoring of volatile organic compounds (VOCs) and plant physiological markers was evaluated using 10 fungal and bacterial pathogens of potato in laboratory-scale experiments. Data analysis of HS-SPME-GC-MS revealed 130 compounds released from infected potatoes, including sesquiterpenes, dimethyl disulfide, 1,2,4-trimethylbenzene, 2,6,11-trimethyldodecane, benzothiazole, 3-octanol, and 2-butanol, which may have been associated with the activity of Fusarium sambucinum, Alternaria tenuissima and Pectobacterium carotovorum. In turn, acetic acid was detected in all infected samples. The criteria of selection for volatiles for possible use as incipient disease indicators were discussed in terms of potato physiology. The established physiological markers proved to demonstrate a negative effect of phytopathogens infecting seed potatoes not only on the kinetics of stem and root growth and the development of the entire root system, but also on gas exchange, chlorophyll content in leaves, and yield. The negative effect of phytopathogens on plant growth was dependent on the time of planting after infection. The research also showed different usefulness of VOCs and physiological markers as the indicators of the toxic effect of inoculated phytopathogens at different stages of plant development and their individual organs.