Quantification of trehalose in biological samples with a conidial trehalase from the thermophilic fungus Humicola grisea var. thermoidea

• Published in: World journal of microbiology & biotechnology Vol. 10; no. 1; pp. 17 - 19
• Main Authors: Neves, M.J, Terenzi, H.F, Leone, F.A, Jorge, J.A
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 1994
• Subjects: Biological and medical sciences; Biotechnology; enzyme activity; Enzyme engineering; Fundamental and applied biological sciences. Psychology; Humicola; Humicola grisea; Methods. Procedures. Technologies; Miscellaneous; rapid methods; trehalase; trehalose; Temperature; Stability; Trehalose; Enzyme; Environmental factor; Method; Fungi; Hydrolysis; Enzymatic activity; α,α-Trehalase; Glycosidases; Microorganism culture; Hydrolases; Fungi Imperfecti; O-Glycosidases; Thallophyta; Quantitative analysis; Conidia

The partially-purified, thermally-stable trehalase from conidia of Humicola grisea was highly specific for trehalose and was free of potentially interfering activities. The enzyme was fully stable when stored in solution at -15°C for at least 6 months. This preparation could be used to quantify treh...