Quantification of trehalose in biological samples with a conidial trehalase from the thermophilic fungus Humicola grisea var. thermoidea

The partially-purified, thermally-stable trehalase from conidia of Humicola grisea was highly specific for trehalose and was free of potentially interfering activities. The enzyme was fully stable when stored in solution at -15°C for at least 6 months. This preparation could be used to quantify treh...

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Published inWorld journal of microbiology & biotechnology Vol. 10; no. 1; pp. 17 - 19
Main Authors Neves, M.J, Terenzi, H.F, Leone, F.A, Jorge, J.A
Format Journal Article
LanguageEnglish
Published Dordrecht Springer 1994
Subjects
Biological and medical sciences
Biotechnology
enzyme activity
Enzyme engineering
Fundamental and applied biological sciences. Psychology
Humicola
Humicola grisea
Methods. Procedures. Technologies
Miscellaneous
rapid methods
trehalase
trehalose
Temperature
Stability
Trehalose
Enzyme
Environmental factor
Method
Fungi
Hydrolysis
Enzymatic activity
α,α-Trehalase
Glycosidases
Microorganism culture
Hydrolases
Fungi Imperfecti
O-Glycosidases
Thallophyta
Quantitative analysis
Conidia
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Summary:The partially-purified, thermally-stable trehalase from conidia of Humicola grisea was highly specific for trehalose and was free of potentially interfering activities. The enzyme was fully stable when stored in solution at -15°C for at least 6 months. This preparation could be used to quantify trehalose from 0.05 to 1.25 μmol/ml either in carbohydrate mixtures or in complex biological materials.
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ISSN:0959-3993
1573-0972
DOI:10.1007/BF00357555