The allergic inflammatory reaction in neonatal streptozotocininduced diabetic rats: evidence of insulin resistance and microvascular dysfunction

• Published in: Inflammation research Vol. 57; no. 11; pp. 535 - 541
• Main Authors: Cavalher-Machado, S. C., Cuman, R. K. N., Sartoretto, J. L., Martins, J. O., de. Lima, W. Tavares, Martins, M. A., Silva, P. M. R., Sannomiya, P.
• Format: Journal Article
• Language: English
• Published: Basel SP Birkhäuser Verlag Basel 01.11.2008; Springer Nature B.V
• Subjects: Allergology; Animals; Animals, Newborn; Biomedical and Life Sciences; Biomedicine; Capillary Permeability; Dermatology; Diabetes Mellitus, Experimental - complications; Glucose Tolerance Test; Hypersensitivity - etiology; Immunology; Inflammation - etiology; Insulin Resistance; Male; Neurology; Ovalbumin - immunology; Pharmacology/Toxicology; Pleurisy - etiology; Rats; Rats, Wistar; Rheumatology; Streptozocin; Type 2 diabetes; Lung vascular permeability; Allergic inflammatory reaction
• ISSN: 1023-3830; 1420-908X
• DOI: 10.1007/s00011-008-8047-0

. Objective: To investigate the allergic reaction in neonatal streptozotocin (nSTZ)-induced diabetes mellitus. Material: Male newborn Wistar rats were made diabetic by the injection of streptozotocin (160 mg/kg, i. p.) and used 8 weeks thereafter. Treatment: Animals were sensitized against ovalbumin (OA, 50 μg and Al(OH)3, 5 mg, s. c.) and challenged 14 or 21 days thereafter. Methods: OA-induced airway inflammation and OA-induced pleurisy models were used to investigate leukocyte migration (total and differential leukocyte counts) and lung vascular permeability (Evans blue dye extravasation). Results: nSTZ-diabetic rats presented glucose intolerance and insulin resistance. Relative to controls, nSTZ rats exhibited a 30% to 50% reduction in lung vascular permeability. Leukocyte infiltration in both models of allergen-induced inflammation, and number of pleural mast cells did not differ between groups. Conclusions: Data suggest that the reduction of allergic inflammatory reactions in nSTZ rats is restricted to microvascular dysfunctions and associated, probably, with insulin resistance in lung microvascular endothelium.