Single-cell profiling and functional screening reveal crucial roles for lncRNAs in the epidermal re-epithelialization of human acute wounds

Re-epithelialization is an important physiological process for repairing skin barrier function during wound healing. It is primarily mediated by coordinated migration, proliferation, and differentiation of keratinocytes. Long noncoding RNAs (lncRNAs) are essential components of the noncoding genome...

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Published inFrontiers in surgery Vol. 11; p. 1349135
Main Authors Xiao, Yunting, Zhang, Chenyang, Liu, Xiuping, Yang, Yong, Landén, Ning Xu, Zhang, Zhao, Li, Dongqing
Format Journal Article
LanguageEnglish
Published Switzerland Frontiers Media S.A 26.02.2024
Subjects
keratinocyte differentiation
long noncoding RNA
Medicin och hälsovetenskap
re-epithelialization
single-cell sequencing
Surgery
wound healing
long noncoding RNA
single-cell sequencing
keratinocyte differentiation
wound healing
re-epithelialization
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Summary:Re-epithelialization is an important physiological process for repairing skin barrier function during wound healing. It is primarily mediated by coordinated migration, proliferation, and differentiation of keratinocytes. Long noncoding RNAs (lncRNAs) are essential components of the noncoding genome and participate in various biological processes; however, their expression profiles and function in re-epithelialization during wound healing have not been established. We investigated the distribution of lncRNAs during wound re-epithelialization by comparing the genomic profiles of uninjured skin and acute wound (AW) from healthy donors. We performed functional screening of differentially expressed lncRNAs to identify the important lncRNAs for re-epithelialization. The expression of multiple lncRNAs is changed during human wound re-epithelialization process. We identified VIM-AS1, SMAD5-AS1, and LINC02581 as critical regulators involved in keratinocyte migration, proliferation, and differentiation, respectively. LncRNAs play crucial regulatory roles in wound re-epithelialization. We established lncRNA expression profile in human acute wounds compared with intact skin, offering valuable insights into the physiological mechanisms underlying wound healing and potential therapeutic targets.
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Reviewed by: Weimin Shen, Children’s Hospital of Nanjing Medical University, China
Abbreviations lncRNAs, long noncoding RNAs; AW, acute wound; DAMPs, damage-associated molecular patterns; LN5, laminin-5’;HB-EGF, heparin-binding EGF-like growth factor; EGF, epidermal growth factor; KGF, keratinocyte growth factor; IGF, insulin-like growth factor; FGFs, fibroblast growth factors; VU, venous ulcer; DFU, diabetic foot ulcer; FAO, fatty acid oxidation; FPKM, fragments per kilobase of transcript sequence per millions base pairs mapped; PCA, principal component analysis; ASOs, antisense oligonucleotides; FLG, filaggrin; LOR, loricrin; IVL, involucrin; KRT10, keratin 10; APC, adenomatous polyposis coli.
Edited by: Zhe Li, Concord Repatriation General Hospital, Australia
These authors share first authorship
Shi-Cong Tao, Shanghai Jiao Tong University, China
ISSN:2296-875X
2296-875X
DOI:10.3389/fsurg.2024.1349135