Cardiological aging in SAM model: effect of chronic treatment with growth hormone

• Published in: Biogerontology (Dordrecht) Vol. 11; no. 3; pp. 275 - 286
• Main Authors: Forman, K., Vara, E., García, C., Ariznavarreta, C., Escames, G., Tresguerres, J. A. F.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.06.2010; Springer; Springer Nature B.V
• Subjects: Aging; Aging - metabolism; Aging - physiology; Alzheimer's disease; Animals; Apoptosis; Base Sequence; Biological and medical sciences; Biomedical and Life Sciences; Cardiovascular system; Cell Biology; Cytokines; Cytokines - metabolism; Development. Metamorphosis. Moult. Ageing; Developmental Biology; DNA Primers; Endothelium; Fundamental and applied biological sciences. Psychology; Geriatrics/Gerontology; Growth Hormone - administration & dosage; Growth hormones; Heart - physiology; Heart failure; Life Sciences; Liver; Male; Mice; Nitric oxide; Oxidative stress; Physiology; Research Article; Reverse Transcriptase Polymerase Chain Reaction; Senescence; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Madrid Spain; Spain; Cardiovascular system; Aging; Oxidative stress; Inflammation; Growth hormone; Apoptosis; Senescence; Ageing; Somatotropin; Vertebrata; Chronic; Mammalia; Treatment; Adenohypophyseal hormone; Models
• ISSN: 1389-5729; 1573-6768
• DOI: 10.1007/s10522-009-9245-z

The purpose of this study was to investigate the effect of aging on different parameters related to inflammation, oxidative stress and apoptosis in hearts from two types of male mice models: senescence-accelerated mice (SAM-P8) and senescence-accelerated-resistant (SAM-R1), and the influence of chronic administration of Growth Hormone (GH) on old SAM-P8 mice. Forty male mice were used. Animals were divided into five experimental groups: two 10 month old untreated groups (SAM-P8/SAM-R1), two 2 month old young groups (SAM-P8/SAM-R1) and one 10 month old group (SAM-P8) treated with GH for 30 days. The expression of tumor necrosis factor-alpha, interleukin 1, interleukin 10, heme oxygenases 1 and 2, endothelial and inducible nitric oxide synthases, NFkB, Bad, Bax and Bcl-2 were determined by real-time reverse transcription polymerase chain reaction (RT-PCR). Results were submitted to a two way ANOVA statistical evaluation using the Statgraphics program. Inflammation, as well as, oxidative stress and apoptosis markers were increased in the heart of old SAM-P8 males, as compared to young controls and this situation was not observed in the old SAM-R1 mice. Exogenous GH administration reverted the effect of aging in the described parameters of old SAM-P8 mice. Our results suggest that inflammation, apoptosis and oxidative stress could play an important role in the observed cardiovascular alterations related to aging of SAM-P8 mice and that GH may play a potential protective effect on the cardiovascular system of these animals.