38 kDa chlorophyll a/b protein of the prokaryote Prochlorothrix hollandica is encoded by a divergent pcb gene

• Published in: Plant molecular biology Vol. 36; no. 5; pp. 709 - 716
• Main Authors: Staay, G.W.M. van der, Yurkova, N, Green, B.R
• Format: Journal Article
• Language: English
• Published: Netherlands Springer Nature B.V 01.03.1998
• Subjects: Amino Acid Sequence; amino acid sequences; Chlorophyll; Chlorophyll - genetics; Chlorophyll A; chlorophyll a/b binding protein; Cyanobacteria; DNA, Bacterial - genetics; duplication; Evolution; Evolution, Molecular; Freshwater; genbank/x97043; gene expression; Genes; Genes, Bacterial; messenger RNA; Models, Genetic; Molecular Sequence Data; Multigene Family; nucleotide sequences; pcba gene; pcbb gene; pcbc gene; Phylogeny; Prochlorothrix - genetics; Prochlorothrix hollandica; Sequence Homology, Amino Acid; Space life sciences; structural genes; transcription (genetics); Transcription, Genetic
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1023/a:1005930210515

The chlorophyll (Chl) a/b proteins of the photosynthetic prokaryotes appear to have evolved by gene duplication and divergence of the core Chl a antenna family, which also includes CP43 and CP47 and the iron-stress induced Chl a-binding IsiA proteins. We show here that Prochlorothrix hollandica has a cluster of three pcb (prochlorophyte chlorophyll b) genes which are co-transcribed. The major antenna polypeptides of 32 and 38 kDa are encoded by pcbA and pcbC respectively. The pcbC gene is significantly divergent from the other two and may have originated by a gene duplication independent of the one that led to isiA and the other prochlorophyte pcb genes. The distant relatedness of the three prochlorophyte genera implies that not only the ability to make Chl b and use it for light-harvesting arose independently in the three lineages, but also that the pcb genes may have arisen as the result of independent gene duplications in each lineage.