Procedures and potential pitfalls for constructing a bee-infecting RNA virus clone
Viruses are factors that can fluctuate insect populations, including honey bees. Most honey bee infecting viruses are single positive-stranded RNA viruses that may not specifically infect honey bees and can be hazardous to other pollinator insects. In addition, these viruses could synergize with oth...
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Published in | Frontiers in insect science Vol. 2; p. 908702 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
26.09.2022
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Subjects | |
Online Access | Get full text |
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Summary: | Viruses are factors that can fluctuate insect populations, including honey bees. Most honey bee infecting viruses are single positive-stranded RNA viruses that may not specifically infect honey bees and can be hazardous to other pollinator insects. In addition, these viruses could synergize with other stressors to worsen the honey bee population decline. To identify the underlying detailed mechanisms, reversed genetic studies with infectious cDNA clones of the viruses are necessary. Moreover, an infectious cDNA clone can be applied to studies as an ideal virus isolate that consists of a single virus species with a uniform genotype. However, only a few infectious cDNA clones have been reported in honey bee studies since the first infectious cDNA clone was published four decades ago. This article discusses steps, rationales, and potential issues in bee-infecting RNA virus cloning. In addition, failed experiences of cloning a Deformed wing virus isolate that was phylogenetically identical to Kakugo virus were addressed. We hope the information provided in this article can facilitate further developments of reverse-genetic studies of bee-infecting viruses to clarify the roles of virus diseases in the current pollinator declines. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 This article was submitted to Insect Health and Pathology, a section of the journal Frontiers in Insect Science Reviewed by: Alexander McMenamin, Oak Ridge Institute for Science and Education (ORISE), United States; Eugene V Ryabov, University of Maryland, United States Edited by: Elke Genersch, Institute for Bee Research Hohen Neuendorf (LIB), Germany |
ISSN: | 2673-8600 2673-8600 |
DOI: | 10.3389/finsc.2022.908702 |