Impact of heavy alcohol consumption on cortical bone mechanical properties in male rhesus macaques

• Published in: Bone (New York, N.Y.) Vol. 181; p. 117041
• Main Authors: Shin, Mihee, Kim, Do Kyung, Jain, Manish, Martens, Penny J., Turner, Russell T., Iwaniec, Urszula T., Kruzic, Jamie J., Gludovatz, Bernd
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.2024
• Subjects: Alcohol; Alcohol Drinking; Animals; Bone; Bone and Bones; Cortical Bone; Crack resistance curve behavior; Deformation and fracture toughness; Ethanol; Macaca mulatta; Male; Mechanical characterization; Microstructure; Non-human primate; Strength; Crack resistance curve behavior; Non-human primate; Alcohol; Bone; Microstructure; Mechanical characterization; Deformation and fracture toughness; Strength
• ISSN: 8756-3282; 1873-2763
• DOI: 10.1016/j.bone.2024.117041

Chronic heavy alcohol consumption may influence the skeleton by suppressing intracortical bone remodeling which may impact the quality of bone and its mechanical properties. However, this aspect has not been thoroughly assessed in either humans or animal models whose cortical bone microstructure resembles the microstructure of human cortical bone. The current study is the first to investigate the effects of chronic heavy alcohol consumption on various mechanical properties of bone in a non-human primate model with intracortical remodeling. Male rhesus macaques (5.3 years old at the initiation of treatment) were induced to drink alcohol and then given the choice to voluntarily self-administer water or ethanol (4 % w/v) for approximately 14 months, followed by three abstinence phases (lasting 34, 41, and 39–46 days) with approximately 3 months of ethanol access in between. During the initial 14 months of open-access, monkeys in the alcohol group consumed an average of 2.9 ± 0.8 g/kg/d ethanol (mean ± SD) resulting in a blood ethanol concentration of 89 ± 47 mg/dl in longitudinal samples taken at 7 h after the daily sessions began. To understand the impact of alcohol consumption on material properties, various mechanical tests were conducted on the distal tibia diaphysis of 2–5 monkeys per test group, including dynamic mechanical analysis (DMA) testing, nano-indentation, microhardness testing, compression testing, and fracture resistance curve (R-curve) testing. Additionally, compositional analyses were performed using Fourier-transform infrared (FTIR) spectroscopy. Significant differences in microhardness, compressive stress-strain response, and composition were not observed with alcohol consumption, and only minor differences were detected in hardness and elastic modulus of the matrix and osteons from nanoindentation. Furthermore, the R-curves of both groups overlapped, with similar crack initiation toughness, despite a significant decrease in crack growth toughness (p = 0.032) with alcohol consumption. However, storage modulus (p = 0.029) and loss factor (p = 0.015) from DMA testing were significantly increased in the alcohol group compared to the control group, while loss modulus remained unchanged. These results indicate that heavy alcohol consumption may have only a minor influence on the material properties and the composition of cortical bone in young adult male rhesus macaques. •First alcohol study investigating bone mechanical properties using rhesus macaques•DMA testing revealed increase in storage modulus and decrease in loss factor•Hardness, compressive stress-strain response, and bone composition were not affected•Crack initiation toughness was unchanged but growth toughness decreased