pH-responsive aggregates consisted of oligodeoxynucleotides bearing nitrophenol group that deliver drugs into acidic cells

• Published in: Bioorganic & medicinal chemistry letters Vol. 58; p. 128519
• Main Authors: Yokota, Shohei, Motohashi, Yuto, Nishihara, Tatsuya, Tanabe, Kazuhito
• Format: Journal Article
• Language: English
• Published: OXFORD Elsevier Ltd 15.02.2022; Elsevier
• Subjects: A549 Cells; Antimetabolites, Antineoplastic - chemistry; Antimetabolites, Antineoplastic - pharmacology; Cell Proliferation - drug effects; Cell Survival - drug effects; Chemistry; Chemistry, Medicinal; Chemistry, Organic; Dose-Response Relationship, Drug; Drug Delivery Systems; Drug Screening Assays, Antitumor; Fluorouracil - chemistry; Fluorouracil - pharmacology; Humans; Hydrogen-Ion Concentration; Life Sciences & Biomedicine; Molecular Structure; Nitrophenols - chemistry; Oligodeoxyribonucleotides - chemistry; Pharmacology & Pharmacy; Physical Sciences; Science & Technology; Structure-Activity Relationship; DNA
• ISSN: 0960-894X; 1464-3405
• DOI: 10.1016/j.bmcl.2021.128519

[Display omitted] A decrease in pH is observed in most solid tumors, thus, the development of drug delivery systems that respond to slightly acidic extracellular pH environment is important in providing tumor-targeted therapies. DNA aggregates can act as useful drug delivery agents, and therefore, we designed an artificial oligodeoxynucleotides (ODNs) that formed an aggregate only under acidic conditions in this study. In other words, we expected that if we could make DNA aggregates that form only in an acidic environment and that encapsulate drugs, it would be possible to transport drugs to tumor tissues selectively. Nitrophenol derivatives, which underwent protonation and deprotonation in response to pH changes, was introduced into ODNs. The ODNs formed aggregates under weakly acidic conditions because of expression of amphiphilicity, which was induced by protonation of nitrophenol unit, and were smoothly taken up into cells. We also found that the aggregates transported anticancer drug, 5FU, into acidified cells to show cytotoxic effects.