Characteristics of retrotransposition Activity of the gtwin retrovirus of the Drosophila melanogaster line G32

Stefanov et al tested a series of D. melanogaster strains for the presence of neighboring gtwin copies by PCR using genomic DNA as a template. The annealing sites for PCR primers were located at both ends of gtwin in the vicinity of its long terminal repeats (LTRs). Synthesis proceeded in the direct...

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Published inDoklady. Biochemistry and biophysics Vol. 424; no. 1; pp. 42 - 45
Main Authors Stefanov, Yu. E., Kotnova, A. P., Glukhov, I. A., Lyubomirskaya, N. V., Ilyin, Y. V.
Format Journal Article
LanguageEnglish
Published Dordrecht SP MAIK Nauka/Interperiodica 01.02.2009
Springer Nature B.V
Subjects
Animals
Base Sequence
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Drosophila melanogaster
Drosophila melanogaster - genetics
Genetics
Genome, Insect
Insects
Life Sciences
Molecular Biology
Molecular Sequence Data
Retroelements
DOKLADY Biochemistry
Precise Excision
Mobile Genetic Element
Minus Strand
Genomic Copy
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Summary:Stefanov et al tested a series of D. melanogaster strains for the presence of neighboring gtwin copies by PCR using genomic DNA as a template. The annealing sites for PCR primers were located at both ends of gtwin in the vicinity of its long terminal repeats (LTRs). Synthesis proceeded in the direction of the genomic environment of the element. They revealed the structures which, with a high probability, are extrachromosomal circular copies of gtwin containing both insertions and deletions between LTRs as well as copies in which normal LTRs are located immediately one after another.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1607-6729
1608-3091
DOI:10.1134/S1607672909010128