Scardovia wiggsiae sp. nov., isolated from the human oral cavity and clinical material, and emended descriptions of the genus Scardovia and Scardovia inopinata

• Published in: International journal of systematic and evolutionary microbiology Vol. 61; no. Pt 1; pp. 25 - 29
• Main Authors: Downes, Julia, Mantzourani, Maria, Beighton, David, Hooper, Samuel, Wilson, Melanie J, Nicholson, Ainsley, Wade, William G
• Format: Journal Article
• Language: English
• Published: England 01.01.2011
• Subjects: Acetic Acid - metabolism; Actinobacteria - classification; Actinobacteria - genetics; Actinobacteria - isolation & purification; Actinobacteria - physiology; Base Composition; Cluster Analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Fatty Acids - analysis; Fermentation; Gram-Positive Bacterial Infections - microbiology; Humans; Lactic Acid - metabolism; Molecular Sequence Data; Mouth - microbiology; Peptidoglycan - chemistry; Phospholipids - analysis; Phylogeny; Quinones - analysis; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.019752-0

Six strains of anaerobic, pleomorphic Gram-positive bacilli, isolated from the human oral cavity and an infected arm wound, were subjected to a comprehensive range of phenotypic and genotypic tests and were found to comprise a homogeneous group. 16S rRNA gene sequence analysis revealed that the isolates were most closely related to Scardovia inopinata CCUG 35729(T) (94.8-94.9 % 16S rRNA gene sequence similarity). The isolates were saccharolytic and produced acetic and lactic acids as end products of fermentation. The major fatty acids were C(16 : 0) (49.8 %) and C(18 : 1)ω9c (35.8 %). Polar lipid analysis revealed a variety of glycolipids, diphosphatidylglycerol, an unidentified phospholipid and an unidentified phosphoglycolipid. No respiratory quinones were detected. The peptidoglycan was of the type A4α L-Lys-Thr-Glu, with L-lysine partially replaced by L-ornithine. The DNA G+C content of one of the strains, C1A_55(T)(,) was 55 mol%. A novel species, Scardovia wiggsiae sp. nov., is proposed to accommodate the six isolates, with the type strain C1A_55(T) (=DSM 22547(T)=CCUG 58090(T)).