Efficient production of 1,3-propanediol from glycerol upon constitutive expression of the 1,3-propanediol oxidoreductase gene in engineered Klebsiella pneumoniae with elimination of by-product formation

• Published in: Bioprocess and biosystems engineering Vol. 36; no. 6; pp. 757 - 763
• Main Authors: Oh, Baek-Rock, Seo, Jeong-Woo, Heo, Sun-Yeon, Luo, Lian Hua, Hong, Won-Kyung, Park, Don-Hee, Kim, Chul-Ho
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.06.2013; Springer Nature B.V
• Subjects: Alcohol Dehydrogenase - biosynthesis; Alcohol Dehydrogenase - genetics; Bacterial Proteins - biosynthesis; Bacterial Proteins - genetics; bacteriophages; Bacteriophages - genetics; Bacteriophages - metabolism; batch fermentation; Biodiesel fuels; Bioengineering; bioprocessing; Biotechnology; Byproducts; Chemistry; Chemistry and Materials Science; Cryoprotective Agents - metabolism; Cryoprotective Agents - pharmacology; engineering; Environmental Engineering/Biotechnology; Fermentation; Food Science; Gene Expression; genes; Genetic engineering; glycerol; Glycerol - metabolism; Glycerol - pharmacology; Hexosyltransferases - biosynthesis; Hexosyltransferases - genetics; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Klebsiella pneumoniae; Klebsiella pneumoniae - genetics; Klebsiella pneumoniae - metabolism; levansucrase; Metabolic Engineering; mutants; Original Paper; Promoter Regions, Genetic; Propylene Glycols - metabolism; Rahnella - enzymology; Rahnella - genetics; Rahnella aquatilis; Viral Proteins - biosynthesis; Viral Proteins - genetics; Glycerol; By-product formation; 1,3-Propanediol; Constitutive expression
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-013-0901-y

In the present study, we developed an efficient method of 1,3-propanediol (1,3-PD) production from glycerol by genetic engineering of Klebsiella pneumoniae AK mutant strains. The proposed approach eliminated by-product formation and IPTG induction resulted in maximal production of 1,3-PD. A series of recombinant strains was designed to constitutively express the dhaB and/or dhaT genes, using the bacteriophage T5 PDE₂₀ promoter and the rho-independent transcription termination signal of the Rahnella aquatilis levansucrase gene. Among these strains, AK/pConT expressing dhaT alone gave the highest yield of 1,3-PD. Fed-batch fermentation resulted in efficient production of 1,3-PD from either pure or crude glycerol, without by-product formation.