Population genetic data of 4 multicopy Y-STR markers in Chinese

• Published in: Legal medicine (Tokyo, Japan) Vol. 47; p. 101788
• Main Authors: Shang, Lei, Ding, Guangshu, Wu, Yuheng, Chen, Shuiqin, Sun, Hui, Bai, Xue, Zhang, Jian, Li, Wanshui
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2020
• Subjects: Discrimination capacity; Expanded haplotype; Forensic science; Gene diversity; Multicopy Y-STR; Paternal identification; Gene diversity; Paternal identification; Forensic science; Discrimination capacity; Multicopy Y-STR; Expanded haplotype
• ISSN: 1344-6223; 1873-4162; 1873-4162
• DOI: 10.1016/j.legalmed.2020.101788

•Four multicopy Y-STRs were characterized and evaluated in China population.•Allele nomenclature and allelic ladder were developed to avoid tying confusion.•Both conservative and expanded methods were applied to call alleles for DYF371.•Haplotype diversity was higher than 0.8 for DYF409S1, DYF411S1 and DYF371. Novel Y chromosomal STR (Y-STR) markers have been continuously discovered during the past decades, promoting the widely application of Y-STRs in the area of forensic science. Here, four multicopy Y-STR markers were focused, including DYF383S1, DYF409S1, DYF411S1 and DYF371, which are rarely reported in China and differ in the number of copies on Y chromosome. Characterization of the markers was performed in population of Hunan province, China, based on sequence analysis. Allele nomenclature and allelic ladder were then developed to avoid the disunity of typing standard. To evaluate their forensic performance, gene diversity of the four loci was investigated in 548 unrelated male individuals from Hunan population. The number of haplotype was analyzed by both conservative (C-type) and expanded approach (E-type) for markers containing more than 2 copies. As detected, there were 7, 9, 13 alleles and 15, 22, 23 haplotypes for DYF383S1, DYF409S1 and DYF411S1, respectively. Thirty-two C-types and 56 E-types were found in DYF371, indicating the highest haplotype diversity (HD) among all tested loci (0.871 and 0.888 for C-type and E-type, respectively). Two other Y-STRs (DYF409S1, DYF411S1) also showed high haplotype diversity (>0.8) in the population. Combining the four loci, discrimination capacity reached 0.505 (C-type) or 0.533 (E-type), and the total HD values exceeded 0.991. The results inferred great potential of the multicopy markers to improve the resolution of paternal identification in China population.