Aromatic DNA adducts in relation to dietary and other lifestyle factors in Spanish adults

• Published in: European food research & technology Vol. 229; no. 4; pp. 549 - 559
• Main Authors: Ibáñez, Raquel, Peluso, Marco, Munnia, Armelle, Piro, Sara, González, Carlos A, Amiano, Pilar, Tormo, M. J, Ardanaz, Eva, Barricarte, Aurelio, Berenguer, Antonio, Chirlaque, M. Dolores, Dorronsoro, Miren, Jakszyn, Paula, Larrañaga, Nerea, Martínez, Carmen, Navarro, Carmen, Quirós, J. Ramón, Sánchez, M. José, Agudo, Antonio
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.08.2009; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Agriculture; Analytical Chemistry; Biological and medical sciences; Biomarkers; Biotechnology; Blood; Cancer; Carcinogens; Chemistry; Chemistry and Materials Science; Deoxyribonucleic acid; Diet; DNA; Epidemiology; Food; Food industries; Food Science; Forestry; Fundamental and applied biological sciences. Psychology; Genetic testing; Labeling; Laboratories; Leukocytes; Lifestyles; Nutrition research; Occupational exposure; Oils & fats; Oncology; Phenols; Polycyclic aromatic hydrocarbons; Population; Questionnaires; Review Paper; Studies; Lifestyle factor; Nutrients; DNA adducts; Polycyclic aromatic hydrocarbons; Food intake; Human; Hydrocarbon; Polycyclic aromatic compound; Feeding; DNA; Adult; Nutrient; Spanish; Adduct
• ISSN: 1438-2377; 1438-2385
• DOI: 10.1007/s00217-009-1089-x

The aim of this study was to assess the relationship between bulky DNA adducts in white blood cells (WBC) and lifestyle factors in a sample from the Spanish cohort of the European Prospective Investigation into Cancer and Nutrition (EPIC). 296 subjects aged between 35 and 64 years, from five regions, were included. Food intake was estimated with a computerized version of dietary history questionnaire. Daily intake of polycyclic aromatic hydrocarbons (PAH) was estimated using a database with information on food content of potential carcinogens. Data on lifestyle and health factors were collected and DNA adducts measured using the nuclease P1 ³²P-postlabelling technique. Geometric means of adducts were similar for men and women (4.11/10⁹ and 3.94/10⁹ nucleotides, respectively). Highest levels of adduct were observed in non-smokers and non-occupationally exposed. Meat intake, oils and fats were associated with higher levels of adducts, but all non-statistically significant. Higher intakes of calcium, sodium and phosphorus were associated with lower adducts levels. Summarising, our study shows that bulky adducts measured by ³²P-postlabelling in DNA from WBC do not correlate with the usual diet of healthy Spanish adults. Although it has been proposed that diet be the main source of PAH in nonsmokers without occupational exposure, DNA adducts do not seem to be suitable biomarkers of dietary PAH in general population.