Improvement of an Automated Sample Injection System for Pillar Array Columns to Increase Analytical Reproducibility
In our previous study, we developed an automatic sample injection system for pillar array columns for quantitative analysis. An autosampler was used to maintain a constant sample injection volume. However, the sample was diluted during injection using the autosampler, thus deteriorating the analytic...
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| Published in | Molecules (Basel, Switzerland) Vol. 27; no. 15; p. 4715 |
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| Main Authors | , , |
| Format | Journal Article |
| Language | English |
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23.07.2022
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| Abstract | In our previous study, we developed an automatic sample injection system for pillar array columns for quantitative analysis. An autosampler was used to maintain a constant sample injection volume. However, the sample was diluted during injection using the autosampler, thus deteriorating the analytical reproducibility. In this study, we have substituted the autosampler with a syringe pump to overcome the abovementioned problem and improve the system. Sample dilution was avoided by filling the entire capillary with the sample at a constant rate. This improved system also increased the analytical reproducibility. In the previous system, the relative standard deviation (RSD) exceeded 17% of the peak height for coumarin dyes. In contrast, the improved system decreased the RSD to the range 1.2–1.8%. The analytical reproducibility was evaluated by using five types of amino acids. The RSD of each peak height was within 3.0%, confirming good reproducibility. These results indicate that the sample injection method developed in this study can be applied to biological sample analyses as a simple quantitative analysis method for pillar array columns. |
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| AbstractList | In our previous study, we developed an automatic sample injection system for pillar array columns for quantitative analysis. An autosampler was used to maintain a constant sample injection volume. However, the sample was diluted during injection using the autosampler, thus deteriorating the analytical reproducibility. In this study, we have substituted the autosampler with a syringe pump to overcome the abovementioned problem and improve the system. Sample dilution was avoided by filling the entire capillary with the sample at a constant rate. This improved system also increased the analytical reproducibility. In the previous system, the relative standard deviation (RSD) exceeded 17% of the peak height for coumarin dyes. In contrast, the improved system decreased the RSD to the range 1.2–1.8%. The analytical reproducibility was evaluated by using five types of amino acids. The RSD of each peak height was within 3.0%, confirming good reproducibility. These results indicate that the sample injection method developed in this study can be applied to biological sample analyses as a simple quantitative analysis method for pillar array columns. |
| Author | Kuroki, Hiroshi Koyama, Hirotaka Tsunoda, Makoto |
| AuthorAffiliation | 2 Technology Research Laboratory, Shimadzu Corporation, Kyoto 619-0237, Japan; h_koyama@shimadzu.co.jp 1 Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113-0033, Japan; kukee0826@yahoo.co.jp |
| AuthorAffiliation_xml | – name: 1 Graduate School of Pharmaceutical Sciences, University of Tokyo, Tokyo 113-0033, Japan; kukee0826@yahoo.co.jp – name: 2 Technology Research Laboratory, Shimadzu Corporation, Kyoto 619-0237, Japan; h_koyama@shimadzu.co.jp |
| Author_xml | – sequence: 1 givenname: Hiroshi surname: Kuroki fullname: Kuroki, Hiroshi – sequence: 2 givenname: Hirotaka surname: Koyama fullname: Koyama, Hirotaka – sequence: 3 givenname: Makoto surname: Tsunoda fullname: Tsunoda, Makoto |
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| Cites_doi | 10.1039/D0AY00556H 10.1016/S0021-9673(98)00497-X 10.15583/jpchrom.2019.022 10.1016/j.foodchem.2015.01.026 10.1021/ac3001836 10.3390/encyclopedia2010041 10.1021/ac070352p 10.1016/j.clinbiochem.2020.02.001 10.1021/ac902491x 10.1021/ac980028h 10.1016/j.foodchem.2021.129013 10.1021/acs.analchem.6b01201 10.1007/s00216-013-7034-7 10.1016/S0021-9673(02)00240-6 10.1038/nature22314 10.1007/978-3-030-78787-5_9 10.1016/j.jpba.2021.114019 10.1093/chromsci/bmr014 10.1007/s00726-016-2248-6 10.1021/ac034345m |
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| Copyright | 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2022 by the authors. 2022 |
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| SubjectTerms | Amino acids Arrays Automation Biomarkers Coumarin Dilution Injection microchip miniaturization on-chip liquid chromatography Quantitative analysis Reproducibility |
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| Title | Improvement of an Automated Sample Injection System for Pillar Array Columns to Increase Analytical Reproducibility |
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