MHC class I allele frequencies in pigtail macaques of diverse origin

• Published in: Immunogenetics (New York) Vol. 58; no. 12; pp. 995 - 1001
• Main Authors: Pratt, Bridget F, O'Connor, David H, Lafont, Bernard A P, Mankowski, Joseph L, Fernandez, Caroline S, Triastuti, Retno, Brooks, Andrew G, Kent, Stephen J, Smith, Miranda Z
• Format: Journal Article
• Language: English
• Published: United States Springer Nature B.V 01.12.2006
• Subjects: Animal models; Animals; Gene Frequency; Genes, MHC Class I; Human immunodeficiency virus 1; Humans; Immune response; Immunology; Macaca nemestrina; Macaca nemestrina - genetics; Macaca nemestrina - immunology; Primates; Simian immunodeficiency virus; T cell receptors
• ISSN: 0093-7711; 1432-1211
• DOI: 10.1007/s00251-006-0164-8

Pigtail macaques (Macaca nemestrina) are an increasingly common primate model for the study of human AIDS. Major Histocompatibility complex (MHC) class I-restricted CD8(+) T cell responses are a critical part of the adaptive immune response to HIV-1 in humans and simian immunodeficiency virus (SIV) in macaques; however, MHC class I alleles have not yet been comprehensively characterized in pigtail macaques. The frequencies of ten previously defined alleles (four Mane-A and six Mane-B) were investigated in detail in 109 pigtail macaques using reference strand-mediated conformational analysis (RSCA). The macaques were derived from three separate breeding colonies in the USA, Indonesia and Australia, and allele frequencies were analysed within and between these groups. Mane-A*10, an allele that restricts the immunodominant SIV Gag epitope KP9, was the most common allele, present in 32.1% of the animals overall, with similar frequencies across the three cohorts. Additionally, RSCA identified a new allele (Mane-A*17) common to three Indonesian pigtail macaques responding to the same Gag CD8(+) T cell epitope. This broad characterization of common MHC class I alleles in more than 100 pigtail macaques further develops this animal model for the study of virus-specific CD8(+) T cell responses.