Lentiviral Gene Transfer to the Nonhuman Primate Brain

• Published in: Experimental neurology Vol. 160; no. 1; pp. 1 - 16
• Main Authors: Kordower, Jeffrey H, Bloch, Jocelyne, Ma, Shuang Y, Chu, Yaping, Palfi, Stéphane, Roitberg, Ben Zion, Emborg, Marina, Hantraye, Philippe, Déglon, Nicole, Aebischer, Patrick
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.11.1999; Elsevier
• Subjects: Animals; beta-Galactosidase - biosynthesis; beta-Galactosidase - genetics; Biological and medical sciences; Biotechnology; Brain - virology; Caudate Nucleus - virology; Feasibility Studies; Fundamental and applied biological sciences. Psychology; Gene therapy; Genes, Reporter; Genes, Synthetic; Genetic Therapy; Genetic Vectors - administration & dosage; Genetic Vectors - genetics; Health. Pharmaceutical industry; Hepatitis B Virus, Woodchuck - genetics; Industrial applications and implications. Economical aspects; Injections; Lac Operon; Lentivirus; Lentivirus - genetics; Lentivirus - isolation & purification; Lentivirus - physiology; Macaca; Macaca mulatta - virology; Male; Mice; Nerve Tissue Proteins - biosynthesis; Nerve Tissue Proteins - genetics; Parkinson Disease, Secondary - therapy; Phosphoglycerate Kinase - genetics; Promoter Regions, Genetic; Putamen - virology; Recombinant Fusion Proteins - biosynthesis; Recombinant Fusion Proteins - genetics; Stereotaxic Techniques; Substantia Nigra - virology; Central nervous system; Monkey; Retroviridae; Lentivirus; Genetic transfer; Virus; Vertebrata; Mammalia; Animal; Primates; Transduction; Gene therapy; Vector; Nigrostriatal pathway; Brain (vertebrata)
• ISSN: 0014-4886; 1090-2430
• DOI: 10.1006/exnr.1999.7178

Lentiviral vectors infect quiescent cells and allow for the delivery of genes to discrete brain regions. The present study assessed whether stable lentiviral gene transduction can be achieved in the monkey nigrostriatal system. Three young adult Rhesus monkeys received injections of a lentiviral vector encoding for the marker gene β galatosidase (βGal). On one side of the brain, each monkey received multiple lentivirus injections into the caudate and putamen. On the opposite side, each animal received a single injection aimed at the substantia nigra. The first two monkeys were sacrificed 1 month postinjection, while the third monkey was sacrificed 3 months postinjection. Robust incorporation of the βGal gene was seen in the striatum of all three monkeys. Stereological counts revealed that 930,218; 1,192,359; and 1,501,217 cells in the striatum were βGal positive in monkeys 1 (n=2) and 3 (n=1) months later, respectively. Only the third monkey had an injection placed directly into the substantia nigra and 187,308 βGal-positive cells were identified in this animal. The injections induced only minor perivascular cuffing and there was no apparent inflammatory response resulting from the lentivirus injections. Double label experiments revealed that between 80 and 87% of the βGal-positive cells were neurons. These data indicate that robust transduction of striatal and nigral cells can occur in the nonhuman primate brain for up to 3 months. Studies are now ongoing testing the ability of lentivirus encoding for dopaminergic trophic factors to augment the nigrostriatal system in nonhuman primate models of Parkinson's disease.