The cutaneous stress response system in three-spined stickleback and European flounder exposed to oxidative stress: Different mode of action

• Published in: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Vol. 285; p. 111493
• Main Authors: Pomianowski, Konrad, Gozdowska, Magdalena, Sokołowska, Ewa, Kulczykowska, Ewa
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.11.2023
• Subjects: 5-methoxytryptamine; Aralkylamine N-acetyltransferase (AANAT); Asmtl; Cortisol; Fish; Hydroxyindole-O-methyltransferase (HIOMT); Melatonin; N-acetylserotonin O-methyltransferase (ASMT); Skin; Melatonin; 5-methoxytryptamine; Hydroxyindole-O-methyltransferase (HIOMT); Fish; Skin; Cortisol; Asmtl; N-acetylserotonin O-methyltransferase (ASMT); Aralkylamine N-acetyltransferase (AANAT)
• ISSN: 1095-6433; 1531-4332
• DOI: 10.1016/j.cbpa.2023.111493

In fish, the skin is directly exposed to multiple environmental stressors and provides the first line of defense against harmful external factors. It turned out that cortisol and melatonin (Mel) are involved in fish cutaneous stress response system (CSRS) similar to mammalian. This study investigates the mode of action of CSRS in two teleost species of different biology and skin characteristics, the three-spined stickleback and the European flounder, after exposure to oxidative stress induced by a potassium dichromate solution. The cutaneous stress response system presents different ways of action in two studied species: Mel concentration increases in the skin of both species, but cortisol concentration increases in the skin only in sticklebacks. Data suggest that stickleback skin cells can produce cortisol. However, cortisol is not involved in the response to oxidative stress in flounders. In stickleback skin, two genes encoding AANAT and ASMT/HIOMT (enzymes involved in Mel synthesis), aanat1a and asmt2, are expressed, but in flounder skin, only one, asmtl. Because gene expression does not change in stickleback skin after exposure to stress, the source of increased Mel is probably outside the skin. A lack of expression of the gene encoding AANAT in flounder skin strongly suggests that Mel is transported to the skin by the bloodstream from other sites of synthesis. Pigment dispersion in the skin after exposure to oxidative stress is found only in sticklebacks. [Display omitted] •Mel concentration increases in the skin of sticklebacks and flounders exposed to oxidative stress.•Mel is not synthesized in flounder's skin.•The source of increased skin Mel in sticklebacks and flounders exposed to oxidative stress is outside the skin.•Cortisol is involved in the response to oxidative stress in sticklebacks but not in flounders.