SERE, a widely dispersed bacterial repetitive DNA element

• Published in: Journal of medical microbiology Vol. 47; no. 6; pp. 489 - 497
• Main Authors: RAJASHEKARA, G, KOEUTH, T, NEVILE, S, BACK, A, NAGARAJA, K. V, LUPSKI, J. R, KAPUR, V
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.06.1998; Society for General Microbiology
• Subjects: Animals; Bacteriological methods and techniques used in bacteriology; Bacteriology; Base Sequence; Biological and medical sciences; Consensus Sequence; DNA Fingerprinting; DNA Primers - genetics; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Fundamental and applied biological sciences. Psychology; Humans; Interspersed Repetitive Sequences; Microbiology; Molecular Sequence Data; Nucleic Acid Hybridization; Operon; Polymerase Chain Reaction - methods; Polymerase Chain Reaction - statistics & numerical data; Reproducibility of Results; Salmonella enteritidis - classification; Salmonella enteritidis - genetics; Salmonella enteritidis - isolation & purification; Salmonella Phages - classification; Salmonella Phages - genetics; Sequence Homology, Nucleic Acid; Serotyping; Performance evaluation; Polymerase chain reaction; Salmonella enteritidis; Repetitive DNA; Analysis method; Typing; Fingerprint method; Bacteria; Enterobacteriaceae
• ISSN: 0022-2615; 1473-5644
• DOI: 10.1099/00222615-47-6-489

1 Department of Veterinary PathoBiology, University of Minnesota, St Paul, MN 55108 2 Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA Corresponding author: Dr V. Kapur. Received May 13, 1997 Accepted October 2, 1997 The presence of a Salmonella serotype Enteritidis repeat element (SERE) located within the upstream regulatory region of the sefABCD operon encoding fimbrial proteins is reported. DNA dot–blot hybridisation analyses and computerised searches of genetic databases indicate that SERE is well conserved and widely distributed throughout the bacterial and archaeal kingdoms. A SERE-based polymerase chain reaction (SERE-PCR) assay was developed to fingerprint 54 isolates of Enteritidis representing nine distinct phage types and 54 isolates of other Salmonella serotypes. SERE-PCR identified five distinct fingerprint profiles among the 54 Enteritidis isolates; no correlation between phage types and SERE-PCR fingerprint patterns was noticed. SERE-PCR was reproducible, rapid and easy to perform. The results of this investigation suggest that the limited heterogeneity of SERE-PCR fingerprint patterns can be utilised to develop serotype- and serogroup-specific fingerprint patterns for isolates of Enteritidis.