Recent advances on protein separation and purification methods

• Published in: Advances in colloid and interface science Vol. 284; no. C; p. 102254
• Main Authors: Liu, Shixiang, Li, Zhihua, Yu, Bing, Wang, Song, Shen, Youqing, Cong, Hailin
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.10.2020; Elsevier
• ISSN: 0001-8686; 1873-3727; 1873-3727
• DOI: 10.1016/j.cis.2020.102254

Protein, as the material basis of vita, is the crucial undertaker of life activities, which constitutes the framework and main substance of human tissues and organs, and takes part in various forms of life activities in organisms. Separating proteins from biomaterials and studying their structures and functions are of great significance for understanding the law of life activities and clarifying the essence of life phenomena. Therefore, scientists have proposed the new concept of proteomics, in which protein separation technology plays a momentous role. It has been diffusely used in the food industry, agricultural biological research, drug development, disease mechanism, plant stress mechanism, and marine environment research. In this paper, combined with the recent research situation, the progress of protein separation technology was reviewed from the aspects of extraction, precipitation, membrane separation, chromatography, electrophoresis, molecular imprinting, microfluidic chip and so on. [Display omitted] •Overview the main aspects of protein separation and purification.•Compare the main methods of protein separation and purification.•Propose the future developments of protein separation and purification.