TWIST1 Overexpression is Associated with Nodal Invasion and Male Sex in Primary Colorectal Cancer

• Published in: Annals of surgical oncology Vol. 16; no. 1; pp. 78 - 87
• Main Authors: Valdés-Mora, Fátima, Gómez del Pulgar, Teresa, Bandrés, Eva, Cejas, Paloma, Ramírez de Molina, Ana, Pérez-Palacios, Rosa, Gallego-Ortega, David, García-Cabezas, Miguel Angel, Casado, Enrique, Larrauri, Javier, Nistal, Manuel, González-Barón, Manuel, García-Foncillas, Jesús, Lacal, Juan Carlos
• Format: Journal Article
• Language: English
• Published: New York Springer New York 2009; Springer Nature B.V
• Subjects: Aged; Blotting, Western; Colorectal cancer; Colorectal Neoplasms - genetics; Colorectal Neoplasms - pathology; Female; Gastrointestinal Oncology; Gene Expression Regulation, Neoplastic; Humans; Immunoenzyme Techniques; Lymph Nodes; Lymphatic Metastasis; Male; Medicine; Medicine & Public Health; Neoplasm Invasiveness; Neoplasm Staging; Nuclear Proteins - genetics; Oncology; Prognosis; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Sex Factors; Surgery; Surgical Oncology; Twist-Related Protein 1 - genetics; Colorectal Cancer; Colorectal Cancer Sample; Normal Colorectal Mucosa; TWIST1 Expression; Oesophageal Squamous Cell Carcinoma
• ISSN: 1068-9265; 1534-4681
• DOI: 10.1245/s10434-008-0166-x

Background TWIST1 is a basic helix–loop–helix (bHLH) transcription factor that has been involved in tumor progression and metastasis in several cancer types, although no evidence has been provided yet on its implication in colorectal carcinogenesis. Methods We examined the expression pattern of TWIST1 messenger RNA (mRNA) in 54 colorectal cancer biopsies compared with each respective adjacent normal mucosa by real-time reverse-transcriptase polymerase chain reaction (RT-PCR) methodology. Results TWIST1 mRNA was found significantly overexpressed in colorectal cancer samples compared to nontumorous colon mucosa ( P  < 0.0001). Receiver operating characteristic (ROC) curve analysis demonstrated that TWIST1 mRNA levels are significantly increased in patients with nodal invasion and, interestingly, a significant correlation with patient sex was also found. Conclusions Evidence for upregulation of TWIST1 mRNA in colorectal cancer is provided, suggesting its implication in the onset of malignant progression of this disease. In addition, significant higher levels of TWIST1 mRNA were found in men than in women, suggesting a possible transcriptional regulation of TWIST1 by sexual hormones. The use of TWIST1 as a new prognostic marker of advanced malignancy, and as a potential therapeutic target in colorectal cancer, is proposed.