Radiation sterilized bone response to dynamic loading

• Published in: Materials Science & Engineering C Vol. 32; no. 6; pp. 1548 - 1553
• Main Authors: Mardas, Marcin, Kubisz, Leszek, Biskupski, Piotr, Mielcarek, Sławomir, Stelmach-Mardas, Marta, Kałuska, Iwona
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.08.2012
• Subjects: Activation energy; Animals; Biomechanical Phenomena - physiology; Biomechanical Phenomena - radiation effects; Bone; Bone and Bones - physiology; Bone and Bones - radiation effects; Bone Transplantation - methods; Cattle; Denaturation temperature; DMA (Dynamic Mechanical Analysis); Femur - physiology; Femur - radiation effects; Irradiation; Radiation; Radiation Dosage; Sterilization - methods; Irradiation; Denaturation temperature; DMA (Dynamic Mechanical Analysis); Bone; Activation energy
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2012.04.041

Allogeneic bone grafts are used on a large scale in surgeries. To avoid the risk of infectious diseases, allografts should be radiation-sterilized. So far, no international consensus has been achieved regarding the optimal radiation dose. Many authors suggest that bone sterilization deteriorates bone mechanical properties. However, no data on the influence of ionizing radiation on bone dynamic mechanical properties are available. Bovine femurs from 2-year old animal were machine cut and irradiated with the doses 10, 15, 25, 35, 45 and 50kGy. Dynamic mechanical analysis was performed at 1–10Hz at the temperature range of 0–350°C in 3-point bending configuration. No statistically significant differences in storage modulus were observed. However, there were significant decreased values of loss modulus between the samples irradiated with doses of 10 (↓14.3%), 15, 45 and 50kGy (↓33.2%) and controls. It was stated that increased irradiation dose decreases the temperature where collagen denaturation process starts and increases the temperature where the collagen denaturation process finishes. It was shown that activation energy of denaturation process is significantly higher for the samples irradiated with the dose of 50kGy (615kJ/mol) in comparison with control samples and irradiation with other doses (100–135kJ/mol). ► We examine changes in the storage modulus and loss modulus of samples irradiated with doses of 10–50kGy. ► We examine changes in the denaturation temperature of samples irradiated with doses of 10–50kGy. ► We examine changes in the activation energy of denaturation process of samples irradiated with doses of 10–50kGy.