Detection and characterization of recombinant DNA in the Roundup Ready ® soybean insert

The genetically modified (GM) Roundup Ready ® soybean event GTS 40-3-2 contains the bacterial gene 5-enol-pyruvylshikimate-3-phosphate synthase. A 534 bp rearrangement of the DNA in the 3 ′ region flanking the functional insert likely occurred as a consequence of the insertion event. The structure o...

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Bibliographic Details
Published inFood control Vol. 15; no. 6; pp. 471 - 478
Main Authors Lau, Lok-Ting, Collins, Richard A, Yiu, Sui-Hing, Xing, Julie, Yu, Albert C.H
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.09.2004
Subjects
detection
feed composition
food composition
molecular sequence data
polymerase chain reaction
product authenticity
Rearrangement
recombinant DNA
Roundup Ready
soybeans
standards of identity
transgenes
transgenic plants
transposons
GM
Roundup Ready
Rearrangement
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Summary:The genetically modified (GM) Roundup Ready ® soybean event GTS 40-3-2 contains the bacterial gene 5-enol-pyruvylshikimate-3-phosphate synthase. A 534 bp rearrangement of the DNA in the 3 ′ region flanking the functional insert likely occurred as a consequence of the insertion event. The structure of the DNA surrounding the insertion has not been fully characterized. A semi-nested PCR method identified the rearranged soybean DNA in samples of raw, partially processed and highly processed food at a level of 0.01%. The 534 bp rearranged segment contained a contiguous portion at least 238 bp long that was also identified in non-GM soybeans. Specific combinations of semi-nested PCR primers differentiated GM and non-GM soybean DNA. These studies confirm that the rearranged DNA originates from the soybean genome and does not involve the introduction of non-soybean genetic material.
ISSN:0956-7135
1873-7129
DOI:10.1016/j.foodcont.2003.03.001