Detection and characterization of recombinant DNA in the Roundup Ready ® soybean insert
The genetically modified (GM) Roundup Ready ® soybean event GTS 40-3-2 contains the bacterial gene 5-enol-pyruvylshikimate-3-phosphate synthase. A 534 bp rearrangement of the DNA in the 3 ′ region flanking the functional insert likely occurred as a consequence of the insertion event. The structure o...
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Published in | Food control Vol. 15; no. 6; pp. 471 - 478 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Ltd
01.09.2004
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Subjects | |
Online Access | Get full text |
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Summary: | The genetically modified (GM) Roundup Ready
® soybean event GTS 40-3-2 contains the bacterial gene 5-enol-pyruvylshikimate-3-phosphate synthase. A 534 bp rearrangement of the DNA in the 3
′ region flanking the functional insert likely occurred as a consequence of the insertion event. The structure of the DNA surrounding the insertion has not been fully characterized. A semi-nested PCR method identified the rearranged soybean DNA in samples of raw, partially processed and highly processed food at a level of 0.01%. The 534 bp rearranged segment contained a contiguous portion at least 238 bp long that was also identified in non-GM soybeans. Specific combinations of semi-nested PCR primers differentiated GM and non-GM soybean DNA. These studies confirm that the rearranged DNA originates from the soybean genome and does not involve the introduction of non-soybean genetic material. |
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ISSN: | 0956-7135 1873-7129 |
DOI: | 10.1016/j.foodcont.2003.03.001 |