Endothelin binding to cultured calf adrenal zona glomerulosa cells and stimulation of aldosterone secretion

• Published in: The Journal of clinical investigation Vol. 84; no. 3; pp. 1032 - 1035
• Main Authors: Cozza, E N, Gomez-Sanchez, C E, Foecking, M F, Chiou, S
• Format: Journal Article
• Language: English
• Published: United States 01.09.1989
• Subjects: aldosterone; Aldosterone - biosynthesis; Animals; Cattle; Cells, Cultured; endothelin; Endothelins; glomerulosa cells; Kinetics; Peptides - metabolism; Peptides - physiology; Rats; Receptors, Cell Surface - analysis; Receptors, Cell Surface - physiology; Vasoconstrictor Agents - metabolism; Vasoconstrictor Agents - physiology; Zona Glomerulosa - metabolism
• ISSN: 0021-9738
• DOI: 10.1172/JCI114226

Endothelins are a group of potent vasoconstrictors whose structure was deduced from genomic DNA. ET-1 was first isolated from culture supernatants from porcine endothelial cells and ET-3 was identified from a rat DNA library. We report on the binding of 125I-ET-1 to zona glomerulosa cells in culture and on its ability to stimulate aldosterone secretion. Cultured calf adrenal zona glomerulosa cells have saturable, high affinity [Kd = 1.00 +/- 0.17 X 10(-10) M (SEM)] receptors which bind ET-1 in a temperature and time dependent manner. Binding was specific and angiotensin II, vasopressin, ANP, BNP, apamin, calcium channel agonists or antagonists did not interact with the receptor. ET-3 displaced 125I-ET-1 from the receptor with a relative potency of 0.39 +/- 0.1% (SEM) that of ET-1. ET-1 incubated with cultured glomerulosa cells stimulated aldosterone secretion in a dose dependent manner but it was less potent than angiotensin II. ET-3 had less than 1% the relative potency of ET-1 stimulating aldosterone secretion. This data suggest that ET-1 is an independent stimulator of aldosterone secretion and we are speculating that it might be important in those situations, like in malignant hypertension, where endothelial damage might result in increased ET-1 production.