SLERT Regulates DDX21 Rings Associated with Pol I Transcription
Dysregulated rRNA synthesis by RNA polymerase I (Pol I) is associated with uncontrolled cell proliferation. Here, we report a box H/ACA small nucleolar RNA (snoRNA)-ended long noncoding RNA (lncRNA) that enhances pre-rRNA transcription (SLERT). SLERT requires box H/ACA snoRNAs at both ends for its b...
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Published in | Cell Vol. 169; no. 4; pp. 664 - 678.e16 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
04.05.2017
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Subjects | |
Online Access | Get full text |
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Summary: | Dysregulated rRNA synthesis by RNA polymerase I (Pol I) is associated with uncontrolled cell proliferation. Here, we report a box H/ACA small nucleolar RNA (snoRNA)-ended long noncoding RNA (lncRNA) that enhances pre-rRNA transcription (SLERT). SLERT requires box H/ACA snoRNAs at both ends for its biogenesis and translocation to the nucleolus. Deletion of SLERT impairs pre-rRNA transcription and rRNA production, leading to decreased tumorigenesis. Mechanistically, SLERT interacts with DEAD-box RNA helicase DDX21 via a 143-nt non-snoRNA sequence. Super-resolution images reveal that DDX21 forms ring-shaped structures surrounding multiple Pol I complexes and suppresses pre-rRNA transcription. Binding by SLERT allosterically alters individual DDX21 molecules, loosens the DDX21 ring, and evicts DDX21 suppression on Pol I transcription. Together, our results reveal an important control of ribosome biogenesis by SLERT lncRNA and its regulatory role in DDX21 ring-shaped arrangements acting on Pol I complexes.
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•SLERT is a box H/ACA snoRNA-ended lncRNA that enhances pre-rRNA transcription•DDX21 forms ring-shaped structures surrounding Pol I and inhibits Pol I transcription•SLERT binds to DDX21 and modulates DDX21 rings to evict their suppression on Pol I•SLERT-DDX21 interactions regulate differential expression of rDNAs
A long non-coding RNA promotes pre-ribosomal RNA transcription by loosening the ring-shaped structure surrounding multiple RNA Pol I complexes formed by RNA helicase DDX21. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0092-8674 1097-4172 |
DOI: | 10.1016/j.cell.2017.04.011 |