Transcriptional Regulation of miR528 by OsSPL9 Orchestrates Antiviral Response in Rice

• Published in: Molecular plant Vol. 12; no. 8; pp. 1114 - 1122
• Main Authors: Yao, Shengze, Yang, Zhirui, Yang, Rongxin, Huang, Yu, Guo, Ge, Kong, Xiangyue, Lan, Ying, Zhou, Tong, Wang, He, Wang, Wenming, Cao, Xiaofeng, Wu, Jianguo, Li, Yi
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 05.08.2019
• Subjects: Antiviral defence; Gene Expression Regulation, Plant - genetics; Gene Expression Regulation, Plant - physiology; MicroRNAs - genetics; MicroRNAs - metabolism; miR528; Oryza - metabolism; Oryza - virology; Plant Diseases - genetics; Plant Proteins - genetics; Plant Proteins - metabolism; Rice; SPL9; Tenuivirus - genetics; Tenuivirus - pathogenicity; miR528; Antiviral defence; SPL9; Rice
• ISSN: 1674-2052; 1752-9867
• DOI: 10.1016/j.molp.2019.04.010

Many microRNAs (miRNAs) are critical regulators of plant antiviral defense. However, little is known about how these miRNAs respond to virus invasion at the transcriptional level. We previously show that defense against Rice stripe virus (RSV) invasion entailed a reduction of miR528 accumulation in rice, alleviating miR528-mediated degradation of L-Ascorbate Oxidase (AO) mRNA and bolstering the antiviral activity of AO. Here we show that the miR528-AO defense module is regulated by the transcription factor SPL9. SPL9 displayed high-affinity binding to specific motifs within the promoter region of miR528 and activated the expression of miR528 gene in vivo. Loss-of-function mutations in SPL9 caused a significant reduction in miR528 accumulation but a substantial increase of AO mRNA, resulting in enhanced plant resistance to RSV. Conversely, transgenic overexpression of SPL9 stimulated the expression of miR528 gene, hence lowering the level of AO mRNA and compromising rice defense against RSV. Importantly, gain in RSV susceptibility did not occur when SPL9 was overexpressed in mir528 loss-of-function mutants, or in transgenic rice expressing a miR528-resistant AO. Taken together, the finding of SPL9-mediated transcriptional activation of miR528 expression adds a new regulatory layer to the miR528-AO antiviral defense pathway. This study reveals that SPL9 displayed high-affinity binding to specific motifs in the promoter region of miR528 gene and activated its transcription. Accordingly, miR528 accumulation reduces the expression of L-Ascorbate Oxidase, a target of miR528, and compromises plant defense against rice stripe virus.