A Dominant Locus, qBSC-1, Controls β Subunit Content of Seed Storage Protein in Soybean (Glycine max (L.) Merri.)

• Published in: Journal of Integrative Agriculture Vol. 13; no. 9; pp. 1854 - 1864
• Main Authors: WANG, Jun, LIU, Lin, GUO, Yong, WANG, Yong-hui, ZHANG, Le, JIN, Long-guo, GUAN, Rong-xia, LIU, Zhang-xiong, WANG, Lin-lin, CHANG, Ru-zhen, QIU, Li-juan
• Format: Journal Article
• Language: English
• Published: National Key Facility for Gene Resources and Genetic Improvement NFCRI/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China 01.09.2014; Elsevier
• Subjects: QTL mapping; soybean seed storage protein; β subunit; soybean seed storage protein; β subunit; QTL mapping
• ISSN: 2095-3119; 2352-3425
• DOI: 10.1016/S2095-3119(13)60579-1

Soybean seed storage protein is one of the most important plant vegetable proteins, and β subunit is of great signiifcance to enhance soybean protein quality and processing property. F2 segregated population and residual heterozygous lines (RHL) derived from the cross between Yangyandou (low level of β subunit) and Zhonghuang 13 (normal level of β subunit) were used for mapping of β subunit content. Our results showed that β subunit content was controlled by a single dominant locus,qBSC-1(β subunit content), which was mapped to a region of 11.9 cM on chromosome 20 in F2 population of 85 individuals. This region was narrowed down to 2.5 cM between BARCSOYSSR_20_0997 and BARCSOYSSR_20_0910 in RHL with a larger population size of 246 individuals. There were 48 predicted genes withinqBSC-1 region based on the reference genome (Glyma 1.0, Williams 82), including the two copies of β subunit coding geneCG4. An InDel marker developed from a thymine (TT) insertion in one copy ofCG4promoter region in Yangyandou cosegregrated with BARCSOYSSR_20_0975 withinqBSC-1 region, suggesting that this InDel marker maybe useful for marker-assisted selection (MAS).