Sequence analysis of a DNA fragment from Buchnera aphidicola (Aphid Endosymbiont) containing the genes dapD-htrA-ilvI-ilvH-ftsL-ftsI-murE

• Published in: Current microbiology Vol. 37; no. 3; pp. 214 - 216
• Main Authors: Thao, M.L. (University of California, Davis, CA.), Baumann, P
• Format: Journal Article
• Language: English
• Published: New York, NY Springer 01.09.1998; Springer Nature B.V
• Subjects: Amino acids; Bacteriology; Biological and medical sciences; Biosynthesis; Buchnera aphidicola; Cell division; Deoxyribonucleic acid; DNA; ENDOFITAS; ENDOPHYTE; ENDOPHYTES; Fundamental and applied biological sciences. Psychology; GENBANK/AF060492; GENE; GENES; Genetics; Microbiology; SCHIZAPHIS GRAMINUM; SIMBIONTES; SYMBIONTS; SYMBIOTE; Genome organization; Insecta; Biosynthesis; Pest; Intracellular symbiosis; Homoptera; Gene; Aphididae; Bacteria; Locus; Isoleucine; Aphidoidea; Symbionte; Buchnera aphidicola; Enzyme; Peptidases; Peptidoglycan; Lysine; Valine; Aminoacid; Arthropoda; DNA; Hydrolases; Invertebrata; Schizaphis graminum; Structural analysis
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s002849900365

Buchnera aphidicola is a prokaryotic endosymbiont of the aphid Schizaphis graminum. One of the endosymbiont's functions is the synthesis of branched-chain amino acids. A 9.7-kilobase B. aphidicola chromosomal DNA fragment was cloned and sequenced and found to contain genes encoding acetohydroxy acid synthase (ilvIH), the first enzyme of the parallel pathway of isoleucine and valine biosynthesis. Previously we have detected ilvC and ilvD, encoding the two other enzymes of this pathway. In addition the DNA fragment contained genes for cell division (ftsL, ftsI), murein biosynthesis (murE), lysine biosynthesis (dapD) and a periplasmic protease (htrA). In these properties B. aphidicola resembles free-living bacteria