Novel liposome-based and in situ gelling artificial tear formulation for dry eye disease treatment

• Published in: Contact lens & anterior eye Vol. 41; no. 1; pp. 93 - 96
• Main Authors: Acar, Duygu, Molina-Martínez, Irene Teresa, Gómez-Ballesteros, Miguel, Guzmán-Navarro, Manuel, Benítez-del-Castillo, José Manuel, Herrero-Vanrell, Rocío
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.02.2018
• Subjects: Animals; Artificial tear; Disease Models, Animal; Dry eye; Dry Eye Syndromes - drug therapy; Dry Eye Syndromes - metabolism; Gels - administration & dosage; In situ gel; Liposome; Liposomes; Lubricant Eye Drops - therapeutic use; Male; Nanotechnology; Ophthalmic Solutions; Rabbits; Tears - chemistry; Liposome; In situ gel; Dry eye; Nanotechnology; Artificial tear
• ISSN: 1367-0484; 1476-5411
• DOI: 10.1016/j.clae.2017.11.004

•A novel liposome-based, in situ gelling artificial tear formulation was developed.•Physicochemical properties, in vitro and in vivo tolerance were evaluated.•The formulation showed suitable properties for topical ophthalmic administration.•Good in vitro and in vivo tolerance was observed.•The novel artificial tear may be beneficial in the treatment of dry eye disease. Artificial tears are widely used in the treatment of dry eye disease, although current formulations do not closely resemble natural tears. The purpose of this study was the design and characterization of a novel in situ gelling artificial tear formulation, containing both lipid and aqueous components, in order to resemble natural tears and replenish the tear film. Liposomes, containing phosphatidylcholine, cholesterol, vitamins A and E, were prepared by the thin-film hydration method. The aqueous phase of the formulation was comprised of gellan gum, hydroxypropyl methylcellulose, levocarnitine, electrolytes (sodium chloride and potassium chloride), trehalose, and borates. The artificial tear was characterized in terms of liposome size, pH, surface tension, and viscosity. In vitro tolerance studies were performed in a human epithelial carcinoma cell line (HeLa) and a murine macrophage cell line (J774). In vivo tolerance was assessed in rabbits. Liposomes presented a unimodal distribution with a mean size of 200.1 ± 4.4 nm. The resulting surface tension was 53.4 ± 1.1 mN/m (at 33 °C) and the pH was 7.6 ± 0.1. The viscosity of the formulation presented a mean value of 4.0 ± 0.1 mPa s within the shear rate interval of 200–1000 s−1 at 33 °C. Cell viability remained higher than 90% in both cell lines. No discomfort or clinical signs were observed in rabbits. The liposome-based and in situ gelling artificial tear formulation presented good tolerance and suitable properties for topical ophthalmic administration. It may be beneficial in the treatment of dry eye disease.