Mitochondrial Isolation and Real-Time Monitoring of MOMP

Isolated model systems have proven to be the standard in the apoptosis field to deconstruct MOMP into individual steps and to study the behavior of a subset of MOMP regulators. Here we describe the method to isolate, JC-1-label, and purify mouse liver mitochondria and subsequently describe how to ut...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1877; p. 121
Main Authors Luna-Vargas, Mark P A, Mohammed, Jarvier N, Gelles, Jesse D, Chipuk, Jerry Edward
Format Journal Article
LanguageEnglish
Published United States 2019
Subjects
BAX
Real-time MOMP measurements
BCL-2 family
Isolated mitochondria
JC-1 labeling
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Summary:Isolated model systems have proven to be the standard in the apoptosis field to deconstruct MOMP into individual steps and to study the behavior of a subset of MOMP regulators. Here we describe the method to isolate, JC-1-label, and purify mouse liver mitochondria and subsequently describe how to utilize the JC-1-labeled mitochondria for real-time MOMP measurements.
ISSN:1940-6029
DOI:10.1007/978-1-4939-8861-7_8