Effect of gold compounds on the activity of adenylyl cyclase in human lymphocyte membranes

• Published in: Arthritis and rheumatism Vol. 35; no. 8; p. 857
• Main Authors: Lazarevic, M B, Yan, K, Swedler, W I, Rasenick, M M, Skosey, J L
• Format: Journal Article
• Language: English
• Published: United States 01.08.1992
• Subjects: Adenylyl Cyclases - metabolism; Auranofin - pharmacology; Aurothioglucose - pharmacology; B-Lymphocyte Subsets - enzymology; B-Lymphocyte Subsets - ultrastructure; Cell Membrane - drug effects; Cell Membrane - enzymology; Cell Membrane - ultrastructure; Colforsin - pharmacology; Gold - metabolism; Gold - pharmacology; Gold Sodium Thiomalate - pharmacology; Humans; Lymphocytes - drug effects; Lymphocytes - enzymology; Lymphocytes - ultrastructure; Penicillamine - pharmacology; Sulfhydryl Compounds - metabolism; T-Lymphocyte Subsets - enzymology; T-Lymphocyte Subsets - ultrastructure
• ISSN: 0004-3591
• DOI: 10.1002/art.1780350803

To determine the effects of aurothioglucose, aurothiomalate, and auranofin on basal and forskolin-activated adenylyl cyclase activity in human total lymphocyte membranes, and in membranes of T and B lymphocyte subsets. Membranes were isolated from human total lymphocytes and T and B cell subsets. The effects of gold compounds on basal and forskolin-stimulated activity of adenylyl cyclase were measured by radioassay. The gold compounds inhibited adenylyl cyclase activity. This inhibitory effect required the presence of both the sulfhydryl ligands and aurous cation. Regulation of lymphocyte adenylyl cyclase by gold compounds represents a potential mode of action of these drugs in rheumatic diseases.