Isolation and identification of a cDNA clone coding for an HLA-DR transplantation antigen alpha-chain
Membrane-bound mRNA was isolated from Raji cells and enriched for message coding for the HLA-DR transplantation antigen alpha-chain by sucrose gradient centrifugation. Double-stranded cDNA was constructed from this mRNA fraction, ligated to plasmid pBR322, and cloned into Escherichia coli. By hybrid...
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Published in | Scandinavian journal of immunology Vol. 16; no. 4; p. 303 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
01.10.1982
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Subjects | |
Online Access | Get more information |
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Summary: | Membrane-bound mRNA was isolated from Raji cells and enriched for message coding for the HLA-DR transplantation antigen alpha-chain by sucrose gradient centrifugation. Double-stranded cDNA was constructed from this mRNA fraction, ligated to plasmid pBR322, and cloned into Escherichia coli. By hybrid selection, a plasmid, pDR-alpha-1, able to hybridize with mRNA coding for the HLA-DR alpha-chain was identified. From the nucleotide sequence of one end of the insert an amino acid sequence was predicted which is identical to part of the amino-terminal sequence of an HLA-DR alpha-chain preparation isolated from Raji cells. This clearly shows that pDR-alpha-1 carries almost the complete message for an HLD-DR alpha-chain. From the nucleotide sequence of this plasmid it will be possible to predict the primary structure of an HLA-DR alpha-chain. |
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ISSN: | 0300-9475 |
DOI: | 10.1111/j.1365-3083.1982.tb00727.x |