Stable transformation of sunflower using Agrobacterium and split embryonic axis explants

• Published in: Plant science (Limerick) Vol. 103; no. 2; pp. 199 - 207
• Main Authors: Malone-Schoneberg, JoBeth, Scelonge, Chris J., Burrus, Monique, Bidney, Dennis L.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 1994; Elsevier Science
• Subjects: Agrobacterium tumefaciens; Agronomy. Soil science and plant productions; Biological and medical sciences; Biotechnology; Embryonic axis explants; Fundamental and applied biological sciences. Psychology; Genetic engineering applications; Genetics and breeding of economic plants; Helianthus annuus L; Meristem transformation; Microprojectile wounding; Plant breeding: fundamental aspects and methodology; Transgenic plants; Agrobacterium tumefaciens; Meristem transformation; Helianthus annuus L; Embryonic axis explants; Microprojectile wounding; Transgenic plants; Embryo; Microprojectile bombardment; Genetic transformation; Enzyme; Transferases; Explant; Transforming DNA; Compositae; Oil plant(vegetal); Kanamycin kinase; Progeny; Helianthus annuus; Dicotyledones; Angiospermae; Bacteria; Transgenic plant; Spermatophyta; Genetic engineering; Rhizobiaceae; Technique; Embryonic axis
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/0168-9452(94)90208-9

Stable sunflower transformation has been achieved using Agrobacterium tumefaciens strain EHA101 carrying the binary plasmid PHP158 and split embryonic axis explants of sunflower genotype SMF3. PHP158 contained a plant expressible NPTII gene controlled by a double CaMV 35S promoter and the nopaline synthase 3′ region. Chimeric, NPTII-positive transformants were obtained following explant culture in the presence of kanamycin. Seed, associated with the NPTII-positive regions of inflorescences, yielded normal, non-chimeric transformed progeny plants. Southern hybridization analysis detected the presence of integrated T-DNA in T 1, T 2 and T 3 generations. The NPTII gene segregated as a dominant character at a single genetic locus in one T 2 population. NPTII activity remained detectable in T 3 progeny.