Generation of Reovirus Core-like Particles in Cells Infected with Hybrid Vaccinia Viruses That Express Genome Segments L1, L2, L3, and S2

• Published in: Virology (New York, N.Y.) Vol. 197; no. 2; pp. 726 - 731
• Main Authors: Xu, Puting, Miller, Sara E., Joklik, Wolfgang K.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.12.1993; Elsevier
• Subjects: Animals; Biological and medical sciences; Chimera; Fundamental and applied biological sciences. Psychology; Genes, Viral; Genome, Viral; L Cells (Cell Line); Mammalian orthoreovirus 3 - genetics; Mammalian orthoreovirus 3 - growth & development; Mammalian orthoreovirus 3 - ultrastructure; Mice; Microbiology; Morphogenesis; Morphology, structure, chemical composition, physicochemical properties; Nucleotidyltransferases - biosynthesis; Recombinant Proteins - biosynthesis; reovirus; Thymidine Kinase - genetics; vaccinia virus; Vaccinia virus - genetics; Viral Core Proteins - biosynthesis; Virology; Chordopoxvirinae; Reovirus 3; Recombinant virus; Orthopoxvirus; Virus like particle; Nucleocapsid; Reoviridae; Reovirus; Virus; Proteins; Vaccinia virus; Poxviridae; Morphology; Structure
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1006/viro.1993.1648

When mouse L fibroblasts are infected with various combinations of recombinant vaccinia viruses possessing thymidine kinase (TK) genes with inserted reovirus genes that encode core components, particles are formed that closely resemble reovirus cores. In cells infected with vaccinia viruses that express reovirus proteins λ1 and σ2, particles are formed that are very similar to reovirus core shells; if, in addition, the cells are also infected with vaccinia virus that expresses protein λ2, particles are formed that also possess the characteristic icosahedrally located projections/spikes that are present on reovirus cores. If, in either case, the cells are also infected with vaccinia virus that expresses the reovirus RNA polymerase, protein λ3, the resultant particles are morphologically identical with those formed in its absence, but also contain protein λ3.