Direct E-DNA sensor of Mycobacterium tuberculosis mutant strain based on new nanocomposite transducer (Fc-ac-OMPA/MWCNTs)

• Published in: Talanta (Oxford) Vol. 184; pp. 475 - 483
• Main Authors: Bizid, Salma, Blili, Saber, Mlika, Rym, Haj Said, Ayoub, Korri-Youssoufi, Hafsa
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.07.2018; Elsevier
• Subjects: Analytical chemistry; Biotechnology; Chemical Sciences; DNA biosensor; Electrochemistry; Life Sciences; Material chemistry; MWCNTs; or physical chemistry; Poly(p-phenylene); Redox oligomer; Theoretical and; Electrochemistry; Redox oligomer; MWCNTs; DNA biosensor; Poly(p-phenylene)
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2018.03.025

Direct DNA sensor based on new nanocomposite materials (Fc-ac-OMPA/MWCNTs) has been investigated. This nanocomposite was formed by combining the redox oligomer “oligo-methoxy-phenyl-acetonitrile” (Fc-ac-OMPA) and the MWCNTs via a simple π-π stacking interaction in the aim to ameliorate the biosensor performance. The redox indicator and the functional groups of the redox oligomer have been used for monitoring the electrochemical behavior and the flexibility for direct covalent attachment of Hepatitis C DNA probe. This nanocomposite shows high performance of DNA hybridization with a detection limit of 0.08 fmol L−1. Moreover, the biosensor was applied for the detection of pathogenic bacterium such as DNA from Mycobacterium tuberculosis strand. Developed biosensor has been able to detect a single nucleotide polymorphism (SNP)T (TCG/ TTG) which confers resistance of M. tuberculosis to rifampicin drug. [Display omitted] •Direct DNA sensor based on new nanocomposite materials has been investigated.•The redox oligomer functional groups have been used for monitoring the direct covalent attachment of the biomolecule.•The nanocomposite has showed an appreciable electrochemical performance and therefore increased the sensitivity of the biosensor.•The biosensor has been applied for the detection of Mycobacterium tuberculosis strand.