Development to the blastocyst stage of immature pig oocytes arrested before the metaphase-II stage and fertilized in vitro

• Published in: Animal reproduction science Vol. 90; no. 3; pp. 307 - 328
• Main Authors: Somfai, Tamás, Kikuchi, Kazuhiro, Medvedev, Sergey, Onishi, Akira, Iwamoto, Masaki, Fuchimoto, Dai-ichiro, Ozawa, Manabu, Noguchi, Junko, Kaneko, Hiroyuki, Ohnuma, Katsuhiko, Sato, Eimei, Nagai, Takashi
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2005
• Subjects: Animals; blastocyst; Blastocyst - physiology; Cells, Cultured; chromosome number; cumulus oophorus; cytoplasm; diploidy; embryogenesis; Embryonic Development; Female; Fertilization in Vitro; food animals; In vitro fertilization; meiosis; Metaphase; Oocytes; Oocytes - cytology; Oocytes - growth & development; Pig blastocysts; sows; Swine; triploidy; In vitro fertilization; Pig blastocysts; Oocytes

In vitro fertilization (IVF) and embryonic development of mature and meiotically arrested porcine oocytes were compared in the present study. After in vitro maturation (IVM) of cumulus-oocyte complexes for 48 h, 75.4% of them extruded a visible polar body (PB). Most of the oocytes with a first polar...