Low-angle x-ray scattering from lyophilized blood constituents

The characteristic nature of low-angle x-ray scattering from biological samples and its dependence on molecular structure is a subject of increasing interest. In this work, low-angle x-ray scattering from lyophilized (freeze-dried) whole blood, haemoglobin (Hb), serum and red blood cell membranes is...

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Bibliographic Details
Published inPhysics in medicine & biology Vol. 46; no. 2; pp. 531 - 539
Main Authors Elshemey, Wael M, Desouky, Omar S, Ashour, Ahmed H
Format Journal Article
LanguageEnglish
Published Bristol IOP Publishing 01.02.2001
Institute of Physics
Subjects
Biological and medical sciences
Biophysical Phenomena
Biophysics
Blood - radiation effects
Cardiovascular system
Freeze Drying
Gamma Rays
Hemoglobins - radiation effects
Humans
In Vitro Techniques
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Models, Theoretical
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Photons
Scattering, Radiation
X-Ray Diffraction
X-Rays
Human
Evaluation
Scattering spectrometry
Red blood cell
X ray irradiation
Blood
Low energy
Plasma membrane
Biological medium
Hemoglobin
Chemical composition
Lyophilic compound
Signal analysis
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Summary:The characteristic nature of low-angle x-ray scattering from biological samples and its dependence on molecular structure is a subject of increasing interest. In this work, low-angle x-ray scattering from lyophilized (freeze-dried) whole blood, haemoglobin (Hb), serum and red blood cell membranes is studied. The scattering profiles of these samples are found to be reproducible and characteristic. A number of characterization parameters are introduced, showing significant differences between the investigated samples. The sensitivity of the scattering profiles of whole blood, Hb and serum towards induced molecular level variations is examined after doses of gamma irradiation of 3, 6 and 9 Gy. The full width at half maximum (FWHM) of the second peak of scattering and the percentage ratio of amplitudes of the first and second peaks (I1/I2)% are found to be the parameters most sensitive to irradiation. For all irradiated samples, it is observed that the FWHM of the second peak is always greater than the control, while the ratio (I1/I2)% is always smaller than the control.
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ISSN:0031-9155
1361-6560
DOI:10.1088/0031-9155/46/2/318