Detection of Antibodies to dsDNA: An Overview of Laboratory Assays

• Published in: Lupus Vol. 15; no. 7; pp. 403 - 407
• Main Authors: Rouquette, AM, Desgruelles, C
• Format: Journal Article
• Language: English
• Published: Thousand Oaks, CA SAGE Publications 01.01.2006; Sage Publications Ltd
• Subjects: Antibodies; Antibodies, Antinuclear - analysis; Antigens; Autoimmune diseases; Disease; Enzyme-Linked Immunosorbent Assay - methods; Fluorescent Antibody Technique - methods; Laboratories; Lupus; Lupus Erythematosus, Systemic - diagnosis; Lupus Erythematosus, Systemic - immunology; Microarray Analysis - methods; Radioimmunoassay - methods; dsDNA antibodies; methods of detection; systemic lupus erythematosus (SLE)
• ISSN: 0961-2033; 1477-0962
• DOI: 10.1191/0961203306lu2324oa

This brief review is focused on different methodologies available for detection of anti-dsDNA antibodies with respect to the best adequacy between biological results of laboratory and clinical significance. A large array of assays has been developed for the measurement of anti-dsDNA. New assays continually introduced have reflected not only technical innovations to avoid difficulties of some assays, but even more with hope to correlate better with systemic lupus erythematosus (SLE), particularly with its clinical course and exacerbations. Finally, with the development of micro arrays technology new insights into the pathophysiology of autoimmune disease processes should be revealed.